机构:[1]Department of Pathology, Xuanwu Hospital, Capital Medical University, Beijing, China医技科室病理科首都医科大学宣武医院[2]Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), Beijing, China[3]Department of anatomy, School of Basic Medicine, Binzhou Medical University, Yantai, China
High-altitude hypoxia-induced oxidative stress and inflammation played an essential role in the incidence and development of liver injury. Salidroside (Sal), a phenylpropanoid glycoside extracted from the plant Rhodiola rosea, has recently demonstrated antioxidant, anti-inflammatory, and antihypoxia properties. Herein, we hypothesized that salidroside may alleviate hypoxia-induced liver injury via antioxidant and antiinflammatory-related pathways. A high-altitude hypoxia animal model was established using hypobaric chamber. Male SD rats were randomly divided into the control group, hypoxia group, control +Sal group, and hypoxia +Sal group. Salidroside treatment significantly inhibited hypoxia-induced increases of serum and hepatic pro-inflammatory cytokines release, hepatic ROS production and MDA contents; attenuated hypoxia-induced decrease of hepatic SOD, CAT, and GSH-Px activities. Furthermore, salidroside treatment also potentiated the activation of Nrf2-mediated anti-oxidant pathway, as indicated by upregulation of n-Nrf2 and its downstream HO-1 and NQO-1. In vitro study found that blocking the Nrf2 pathway using specific inhibitor ML385 significantly reversed the protective effect of salidroside on hypoxia-induced liver oxidative stress. In addition, salidroside treatment significantly inhibited hepatic pro-inflammatory cytokines release via JAK2/STAT3-mediated pathway. Taken together, our findings suggested that salidroside protected against hypoxia-induced hepatic oxidative stress and inflammation via Nrf2 and JAK2/STAT3 signaling pathways.
基金:
National Natural Science Foundation of China (NSFC) [81700760]; Natural Science Foundation of Shandong Province [ZR2018QH002]
第一作者机构:[1]Department of Pathology, Xuanwu Hospital, Capital Medical University, Beijing, China[2]Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), Beijing, China
通讯作者:
通讯机构:[1]Department of Pathology, Xuanwu Hospital, Capital Medical University, Beijing, China[*1]Department of Pathology, Xuanwu Hospital Capital Medical University, No. 45, Changchun Street, Xicheng District, Beijing 100053, China
推荐引用方式(GB/T 7714):
Xiong Yanlei,Wang Yueming,Xiong Yanlian,et al.Protective effect of Salidroside on hypoxia-related liver oxidative stress and inflammation via Nrf2 and JAK2/STAT3 signaling pathways[J].FOOD SCIENCE & NUTRITION.2021,9(9):5060-5069.doi:10.1002/fsn3.2459.
APA:
Xiong, Yanlei,Wang, Yueming,Xiong, Yanlian&Teng, Lianghong.(2021).Protective effect of Salidroside on hypoxia-related liver oxidative stress and inflammation via Nrf2 and JAK2/STAT3 signaling pathways.FOOD SCIENCE & NUTRITION,9,(9)
MLA:
Xiong, Yanlei,et al."Protective effect of Salidroside on hypoxia-related liver oxidative stress and inflammation via Nrf2 and JAK2/STAT3 signaling pathways".FOOD SCIENCE & NUTRITION 9..9(2021):5060-5069