机构：[1]Shenzhen Key Laboratory of Drug Addiction, Shenzhen Neher Neural Plasticity Laboratory, The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.[2]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.内科系统老年医学科首都医科大学宣武医院[3]University of Chinese Academy of Sciences, Beijing 100049, China.[4]Medical College of Guangxi University, Nanning 530003, China.[5]CAS Key Laboratory of Brain Connectome and Manipulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.[6]Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China.深圳市康宁医院深圳医学信息中心[7]Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.
Parkinson's disease (PD) is a neurodegenerative disorder that features motor and non-motor deficits. The use of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopamine neuron degeneration has been widely practiced to produce reliable animal models of PD. However, most previous preclinical studies focused on motor dysfunction, and few non-motor symptoms were evaluated. Thus far, there is a lack of comprehensive investigations of the non-motor symptoms in animal models.In this study, we aim to use a battery of behavioral methods to evaluate non-motor symptoms in MPTP-induced non-human primate PD models.Cognitive function, sleep, and psychiatric behaviors were evaluated in MPTP-treated cynomolgus monkeys. The tests consisted of a delayed matching-to-sample (DMTS) task, the use of a physical activity monitor (PAM), an apathy feeding task (AFT), the human intruder test (HIT), novel fruit test (NFT), and predator confrontation test (PCT). In addition, we tested whether the dopamine receptor agonist pramipexole (PPX) can improve these non-motor symptoms.The present results show that the MPTP-treated monkeys exhibited cognitive deficits, abnormal sleep, and anxiety-like behaviors when compared to the control monkeys. These symptoms were relieved partially by PPX.These results suggest that MPTP-induced PD monkeys displayed non-motor symptoms that were similar to those found in PD patients. PPX treatment showed moderate therapeutic effects on these non-motor symptoms. This battery of behavioral tests may provide a valuable model for future preclinical research.
基金：
This work was supported in part by the STI2030-Major Projects (2021ZD0200900), the
National Key Research and Development Project of China (2018YFA0108503, 2018YFA0108304),
the National Natural Science Foundation of China (U20A2017), Shenzhen Science and Technology
Innovation Commission (ZDSYS20190902093601675), the Shenzhen Science and Technology Program
(KQTD20210811090117032), and the National Hainan Key Research and Development Project
(ZDYF2021SHFZ049).
第一作者机构：[1]Shenzhen Key Laboratory of Drug Addiction, Shenzhen Neher Neural Plasticity Laboratory, The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.[2]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.[3]University of Chinese Academy of Sciences, Beijing 100049, China.
通讯作者：
通讯机构：[1]Shenzhen Key Laboratory of Drug Addiction, Shenzhen Neher Neural Plasticity Laboratory, The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.[2]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital Medical University, Beijing 100053, China.[5]CAS Key Laboratory of Brain Connectome and Manipulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.[6]Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China.[7]Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, China.
推荐引用方式(GB/T 7714)：
Bao Yu,Gan Chaoning,Chen Zuyue,et al.Quantification of Non-Motor Symptoms in Parkinsonian Cynomolgus Monkeys[J].BRAIN SCIENCES.2023,13(8):doi:10.3390/brainsci13081153.
APA：
Bao Yu,Gan Chaoning,Chen Zuyue,Qi Zhongquan,Meng Zhiqiang&Yue Feng.(2023).Quantification of Non-Motor Symptoms in Parkinsonian Cynomolgus Monkeys.BRAIN SCIENCES,13,(8)
MLA：
Bao Yu,et al."Quantification of Non-Motor Symptoms in Parkinsonian Cynomolgus Monkeys".BRAIN SCIENCES 13..8(2023)
