详情页 - 首都医科大学宣武医院知识库

当前位置：首页 > 详情页

Sleep deprivation from mid-gestation leads to impaired of motor coordination in young offspring mice with microglia activation in the cerebellar vermis

文献详情

资源类型：

Pubmed体系：

作者：

机构： [1]Department of Child and Adolescent Health, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China [2]Department of Pathology, First Hospital of Shanxi Medical University, Taiyuan, 030001, China [3]Department of Basic Medicine, Fenyang College of Shanxi Medical University, Luliang, 032299, China [4]Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China [5]Division of Neuropsychiatry and Psychosomatics, Department of Neurology, Xuanwu Hospital, National Center for Neurological Disorders, National Clinical Research Center for Geriatric Diseases, Capital Medical University, Beijing, 100053 China [6]Beijing Institute of Brain Disorders, Beijing, 100069, China [7]Institute of Special Medical Sciences, School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, China [8]Key Laboratory of Coal Environmental Pathogenicity and Prevention, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, China [9]Center for Early Childhood Development, Shanxi Medical University, Taiyuan, 030001, China

出处：

DOI：

ISSN：

关键词： Sleep deprivation Offspring Cerebellar vermis Motor coordination Microglia activation Inflammation response

摘要：

To investigate the effects of mid-pregnancy sleep deprivation (SD) in C57BL/6 J mice on the motor coordination of the offspring and to explore the potential mechanism of microglia activation in the cerebellar vermis of the offspring involved in the induction of impaired motor coordination development.C57BL/6 J pregnant mice were randomly divided into the SD and control groups. SD was implemented by the multi-platform method from first day of the middle pregnancy (gestation day 8, GD8). At postnatal day 21 (PND21), we measured the development of motor behavior and collected cerebellar vermis tissues to observe the activation of microglia by H&E staining, the expression of microglia-specific markers ionized calcium-binding adaptor molecule-1 (Iba-1) and cluster of differentiation 68 (CD68) by immunohistochemical, and interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor -α (TNF-α) by real-time quantitative PCR (RT-qPCR).In the offspring of SD group, comparing to the control group, the total time of passage and the reverse crawl distance in the balance beam test, and the frequency of falls from the suspension cord was increased; with lower max rotational speed and shorter duration in the rotarod experiment. Further, we found that the microglia of cerebellar vermis tissues emerged an amoeba-like activation. The mean gray value of Iba-1 was lower, the density of positive cells of CD68 and the expression levels of IL-6 and TNF-α were increased.The motor coordination of offspring is impaired, accompanying a SD from mid-pregnancy, and the cerebellar vermis showed microglia activation and pro-inflammatory response. It suggested the adverse effects of SD from mid-gestation on the development of motor coordination through the inflammatory response in the cerebellar vermis of the offspring.Copyright © 2024 Elsevier B.V. All rights reserved.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2023]版：

大类 | 2 区医学

小类 | 2 区临床神经病学

最新[2023]版：

大类 | 2 区医学

小类 | 2 区临床神经病学

第一作者：

第一作者机构： [1]Department of Child and Adolescent Health, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China

共同第一作者：

通讯作者：

通讯机构： [1]Department of Child and Adolescent Health, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China [5]Division of Neuropsychiatry and Psychosomatics, Department of Neurology, Xuanwu Hospital, National Center for Neurological Disorders, National Clinical Research Center for Geriatric Diseases, Capital Medical University, Beijing, 100053 China [6]Beijing Institute of Brain Disorders, Beijing, 100069, China [7]Institute of Special Medical Sciences, School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, China [8]Key Laboratory of Coal Environmental Pathogenicity and Prevention, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, China [9]Center for Early Childhood Development, Shanxi Medical University, Taiyuan, 030001, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

Sleep deprivation from mid-gestation leads to impaired of motor coordination in young offspring mice with microglia activation in the cerebellar vermis

文献详情

相关文献