机构：[1]Department of Public Health, International College, Krirk University, Bangkok, Thailand.[2]Center for Translational Medicine and Department of Laboratory Medicine, The Third People's Hospital of Longgang District, Shenzhen, China.深圳市康宁医院深圳医学信息中心[3]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Baotou, China.内蒙古科技大学包头医学院[4]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.科技平台低氧适应转化医学北京市重点实验室首都医科大学宣武医院[5]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Baotou, China.内蒙古科技大学包头医学院
Neuroblastoma (NB) is a malignant tumor primarily found in children, presenting significant challenges in its development and prognosis. The role of necroptosis in the pathogenesis of NB has been acknowledged as crucial for treatment. This study aimed to investigate the key genes and functional pathways associated with necroptosis, as well as immune infiltration analysis, in NB. Furthermore, we aimed to evaluate the diagnostic significance of these genes for prognostic assessment and explore their potential immunological characteristics.The NB dataset (GSE19274, GSE73517, and GSE85047) was obtained from the Gene Expression Omnibus (GEO) database, and genes associated with necroptosis were collected from GeneCards and previous literature. First, we conducted differential expression analysis and performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). We employed gene set enrichment analysis (GSEA) to identify overlapping enriched functional pathways from the NB dataset. In addition, we constructed a protein-protein interaction (PPI) network, predicting relevant microRNAs (miRNAs) and transcription factors (TFs), as well as their corresponding drug predictions. Furthermore, the diagnostic value was assessed using receiver operating characteristic (ROC) curves. Finally, an immune infiltration analysis was performed.We identified six necroptosis-related differentially expressed genes (NRDEGs) closely associated with necroptosis in NB. They were enriched in Tuberculosis, Apoptosis-multiple species, Salmonella infection, legionellosis, and platinum drug resistance. GSEA and PPI network analyses, along with mRNA-drug interaction network, revealed 38 potential drugs corresponding to BIRC2, CAMK2G, CASP3, and IL8. ROC curve analysis showed that in GSE19274, FLOT2 with area under the ROC curve (AUC) of 0.850 and DAPK1 with AUC of 0.789.Our study elucidates the key genes and functional pathways associated with necroptosis in NB, offering valuable insights to enhance our comprehension of the pathogenesis of NB, and improve prognosis assessment.2024 Translational Cancer Research. All rights reserved.
基金：
This study was jointly supported by the National
Natural Science Foundation of China (Nos. 81660307,
82060337, 82071479, 31860307), Shenzhen Longgang
District Economic and Technological Development
Special Fund Medical and Health Technology Plan Project
(Nos. LGKCYLWS2021000033, LGKCYLWS2023025),
Shenzhen Science and Technology Plan Project (Nos.
CYJ20220531092412028, JCYJ20230807121306012), the
Inner Mongolia Science Foundation (Nos. 2020MS08010,
2020MS08070).
第一作者机构：[1]Department of Public Health, International College, Krirk University, Bangkok, Thailand.
通讯作者：
通讯机构：[1]Department of Public Health, International College, Krirk University, Bangkok, Thailand.[2]Center for Translational Medicine and Department of Laboratory Medicine, The Third People's Hospital of Longgang District, Shenzhen, China.[3]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Baotou, China.[4]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.[5]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Baotou, China.[*1]Department of Public Health, International College, Krirk University, No. 3 soi Ramintra 1, Ramintra Road, Anusaowaree, Bangkhen, Bangkok 10220, Thailand, Center for Translational Medicine and Department of Laboratory Medicine, The Third People’s Hospital of Longgang District, No. 278 Songbai Road, Henggang Street, Longgang District, Shenzhen 518112, China, Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, No. 31, Jianshe Road, Donghe District, Baotou 014040, China, Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, No. 45, Changchun Street, Xicheng District, Beijing 100053, China[*2]Department of Public Health, International College, Krirk University, No. 3 soi Ramintra 1, Ramintra Road, Anusaowaree, Bangkhen, Bangkok 10220, Thailand ,Center for Translational Medicine and Department of Laboratory Medicine, The Third People’s Hospital of Longgang District, No. 278 Songbai Road, Henggang Street, Longgang District, Shenzhen 518112, China ,Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, No. 31, Jianshe Road, Donghe District, Baotou 014040, China ,Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, No. 45, Changchun Street, Xicheng District, Beijing 100053, China ,Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, No. 41, Lin Yin Road, Kundulun District, Baotou 014017, China
推荐引用方式(GB/T 7714)：
Gao Bing,Yan Shaochun,Xie Wei,et al.Bioinformatics reveals the potential mechanisms and biomarkers of necroptosis in neuroblastoma[J].Translational Cancer Research.2024,13(7):3599-3619.doi:10.21037/tcr-24-14.
APA：
Gao Bing,Yan Shaochun,Xie Wei&Shao Guo.(2024).Bioinformatics reveals the potential mechanisms and biomarkers of necroptosis in neuroblastoma.Translational Cancer Research,13,(7)
MLA：
Gao Bing,et al."Bioinformatics reveals the potential mechanisms and biomarkers of necroptosis in neuroblastoma".Translational Cancer Research 13..7(2024):3599-3619
