The calibration of binocular infrared camera is a key step to realize the positioning of infrared camera. The existing methods of binocular camera calibration are not suitable for infrared camera, so it is necessary to study the methods of binocular infrared camera calibration and positioning. In this paper, a calibration and positioning method of binocular infrared camera is proposed, and the infrared positioning of two kinds of objects is completed by using this method. Firstly, a special temperature calibration system is used to collect the images for binocular calibration, then the center point detection algorithm and Zhang's calibration method are used to process the infrared calibration image to obtain the internal and external parameters of the binocular infrared camera. The calibration error is compared with that of binocular visible camera to prove the feasibility of the binocular infrared camera calibration. Secondly, according to the calibrated parameter matrix of the binocular infrared camera, symmetrical circle calibration object detection algorithm and coordinate transformation are carried out for the distortion correction images to achieve infrared positioning of different objects, and the positioning error is obtained. The results show that the calibration method of binocular infrared camera is feasible, and it has more functions than the traditional calibration method.