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Ependymal cilia decline and AQP4 upregulation in young adult rats with syringomyelia

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机构： [1]Department of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China. [2]Cell Therapy Center, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China. [3]Spine Center, China International Neuroscience Institute (CHINA-INI), Beijing, China. [4]Lab of Spinal Cord Injury and Functional Reconstruction, Xuanwu Hospital, Capital Medical University, Beijing, China. [5]Research Center of Spine and Spinal Cord, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China. [6]National Center for Neurological Disorders, Beijing, China.

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关键词： Cilia Ependymal cell Spinal cord injury Syringomyelia Rat Neuronal necrosis

摘要：

Syringomyelia was a rare condition characterized by the formation of fluid-filled cysts (syrinx) within the spinal cord, resulting in sensory and motor dysfunction. Currently, there was no satisfactory treatment for syringomyelia. Ependymal cells were integral to water transport and may represent a promising therapeutic target.Induction of syringomyelia occurred in 8-week old female rats followed by histological analyses at 3-, 7-, 14-, 30-, 60-, 180-, and 365-days later. Scanning electron microscope (SEM) and transmission electron microscope (TEM) were performed to visualize cilia on rat central canal membrane cells at 30-day post-induction. Syringomyelia was induced via compression at T12-T13 using a sterile cotton ball. Each rat underwent MRI scanning one day before induction and one day prior to sacrifice. In vivo magnetic resonance imaging (MRI) was utilized to measure syrinx enlargement in eight-week-old syringomyelia rats. Histological Analysis and immunofluorescence staining were performed for changes of cilia, neurons, expression of AQP4 and infiltration of immune cells into spinal tissue.In the current study, the cell junctions between ependymal cells of syringomyelia rats were absent, and the cilia on ependymal cells were reduced significantly on day 30 post syringomyelia. The number of ependymal cells kept increasing lasting for 1-2 months and begin to decrease. Edema and vacuolation in the spinal cord tissue are significant in syringomyelia rats. Furthermore, AQP4 expression was elevated in astrocytes of syringomyelia rats, and IBA1+ immune cells infiltrated spinal tissue. Furthermore, neuronal necrosis began in the acute stage of syringomyelia, and reached its peak one month later. Pathological changes in axonal rupture at anterior commissure (connection of the left and right white matter) could be observed in syringomyelia spinal tissue.These findings underscored the significance of cilia on ependymal cells and the evolving microenvironment post-syringomyelia, providing valuable insights for clinical treatment strategies for this condition.© 2025. The Author(s).

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大类 | 1 区医学

小类 | 2 区神经科学

第一作者：

第一作者机构： [1]Department of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China.

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通讯作者：

通讯机构： [1]Department of Neurosurgery, China International Neuroscience Institute, Xuanwu Hospital Capital Medical University, 45 Changchun Street, Beijing, 100053, China. [3]Spine Center, China International Neuroscience Institute (CHINA-INI), Beijing, China. [4]Lab of Spinal Cord Injury and Functional Reconstruction, Xuanwu Hospital, Capital Medical University, Beijing, China. [5]Research Center of Spine and Spinal Cord, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China. [6]National Center for Neurological Disorders, Beijing, China.

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Ependymal cilia decline and AQP4 upregulation in young adult rats with syringomyelia

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