机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.[2]Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham 35294, Alabama, USA.[3]Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.[4]Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou 215000, China
Background: Deep vein thrombosis (DVT) is caused by blood clotting in the deep veins. Thrombosis resolution and recanalization can be accelerated by endothelial progenitor cells. In this report, we investigated the effects of miR-126-loaded EPC-derived exosomes (miR-126-Exo) on EPCs function and venous thrombus resolution. Methods: In vitro promotional effect of miR-126-Exo on the migration and tube incorporation ability of EPCs was investigated via transwell assay and tube formation assay. In addition, a mouse venous thrombosis model was constructed and treated with miR-126-Exo to clarify the therapeutic effect of miR-126-Exo by histological analysis. Lastly, this study predicted a target gene of miR-126 using target prediction algorithms and confirmed it by luciferase activity assay, RT-qPCR, and Western blot. Results: Transwell assay and tube formation assay indicated that miR-126-Exo could enhance the migration and tube incorporation ability of EPCs. Moreover, in vivo study manifested enhanced thrombus organization and recanalization after miR-126-Exo treatment. Meanwhile, we identified that Protocadherin 7 as a target gene of miR-126. Conclusions: To sum up, our results demonstrated that EPC-derived exosomes loaded with miR-126 significantly promoted thrombus resolution in an animal model of venous thrombosis, indicating exosomes as a promising potential vehicle carrying therapeutic molecules for DVT therapy.
基金：
This work was supported by the Natural Science Foundation of Jiangsu
Province (BK20151212 and BK20160346), National Natural Science Foundation
of China (No. 81770260, No. 81400199 and No. 81600217), Jiangsu Province’s
Key Discipline/Laboratory of Medicine (XK201118), and Jiangsu Clinical Research
Center for Cardiovascular Surgery (BL201451).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区医学：研究与实验3 区细胞生物学
最新[2023]版：
大类|2 区医学
小类|2 区细胞与组织工程2 区细胞生物学2 区医学：研究与实验
JCR分区：
出版当年[2016]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ2CELL BIOLOGY
最新[2023]版：
Q1CELL & TISSUE ENGINEERINGQ1CELL BIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[4]Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou 215000, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China.[2]Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham 35294, Alabama, USA.[4]Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou 215000, China
推荐引用方式(GB/T 7714)：
Jiacheng Sun,Zhiwei Zhang,Teng Ma,et al.Endothelial progenitor cell-derived exosomes, loaded with miR-126, promoted deep vein thrombosis resolution and recanalization[J].STEM CELL RESEARCH & THERAPY.2018,9(1):223.doi:10.1186/s13287-018-0952-8.
APA：
Jiacheng Sun,Zhiwei Zhang,Teng Ma,Ziying Yang,Jinlong Zhang...&Qingyou Meng.(2018).Endothelial progenitor cell-derived exosomes, loaded with miR-126, promoted deep vein thrombosis resolution and recanalization.STEM CELL RESEARCH & THERAPY,9,(1)
MLA：
Jiacheng Sun,et al."Endothelial progenitor cell-derived exosomes, loaded with miR-126, promoted deep vein thrombosis resolution and recanalization".STEM CELL RESEARCH & THERAPY 9..1(2018):223
医学