机构：[1]Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, China[2]Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[3]State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210009, China[4]Department of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China[5]Suzhou Key Laboratory of Biomaterials and Technologies & Collaborative Innovation Center, Suzhou Nano Science and Technology, Suzhou 215123, China
The topographical features and material composition of scaffolds have a powerful influence on cell behaviors such as proliferation and differentiation. Here, scaffolds consisting of aligned fibers with incorporated: bioactive collagen I were tested for their ability to enhance osteogenesis in vitro. Rat adipose, derived mesenchymal stem cells (ADSCs) were seeded on the scaffolds and their morphology, proliferation, and osteogenic differentiation ware examined. Aligned scaffolds with collagen I showed the best osteogenic properties. Also, adhesion-related genes showed the higher expression on aligned scaffolds with collagen I. Our findings indicate that fiber alignment combined with incorporation of collagen I increases the capacity of electrospun scaffolds to induce enhanced and directed osteogenesis. Such scaffolds may, therefore, have potential for improving guided oral bone regeneration.
基金：
This work was supported by grants from the State Key
Research and Development Project, China (2016YFA0201704/
2016YFA0201700), Outstanding Medical Academic Leader
Program and Creative Team of Jiangsu Province, the Priority
Academic Program Development of Jiangsu Higher Education
Institutions (PAPD, 2014-37), the National Natural Science
Foundation of China (31200757 and 81400486), the Natural
Science Foundation of Jiangsu Province (BK20140911), Science and the Technology Project in Suzhou, China
(SYG201210), and Science and Development Foundation of
Nanjing Medical University, China (2015NJMU037)
第一作者机构：[1]Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, China
通讯作者：
通讯机构：[2]Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[5]Suzhou Key Laboratory of Biomaterials and Technologies & Collaborative Innovation Center, Suzhou Nano Science and Technology, Suzhou 215123, China
推荐引用方式(GB/T 7714)：
Hanbang Chen,Yunzhu Qian,Yang Xia,et al.Enhanced Osteogenesis of ADSCs by the Synergistic Effect of Aligned Fibers Containing Collagen I[J].ACS APPLIED MATERIALS & INTERFACES.2016,8(43):29289-29297.doi:10.1021/acsami.6b08791.
APA：
Hanbang Chen,Yunzhu Qian,Yang Xia,Gang Chen,Yun Dai...&Ning Gu.(2016).Enhanced Osteogenesis of ADSCs by the Synergistic Effect of Aligned Fibers Containing Collagen I.ACS APPLIED MATERIALS & INTERFACES,8,(43)
MLA：
Hanbang Chen,et al."Enhanced Osteogenesis of ADSCs by the Synergistic Effect of Aligned Fibers Containing Collagen I".ACS APPLIED MATERIALS & INTERFACES 8..43(2016):29289-29297
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