机构：[a]Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China[b]Department of Internal Medicine Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China[c]Department of Radiotherapy and Oncology, the Second Affiliated Hospital of Soochow University, Suzhou 21500, China[d]Department of Stereotactic and Functional Neurosurgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Tumor cell invasion and proliferation remain the overwhelming causes of death for malignant glioma patients. To establish effective therapeutic methods, new targets implied in these processes have to be identified. Tetraspanin 8 (Tspn8) forms complexes with a large variety of trans-membrane and/or cytosolic proteins to regulate several important cellular functions. In the current study, we found that Tspn8 was over-expressed in multiple clinical malignant glioma tissues, and its expression level correlated with the grade of tumors. Tspn8 expression in malignant glioma cells (U251MG and U87MG lines) is important for cell proliferation and migration. siRNA-mediated knockdown of Tspn8 markedly reduced in vitro proliferation and migration of U251MG and U87MG cells. Meanwhile, Tspn8 silencing also increased the sensitivity of temozolomide (TMZ), and significantly increased U251MG or U87MG cell death and apoptosis by TMZ were achieved with Tspn8 knockdown. We observed that Tspn8 formed a complex with activated focal adhesion kinase (FAK) in both human malignant glioma tissues and in above glioma cells. This complexation appeared required for FAK activation, since Tspn8 knockdown inhibited FAK activation in U251MG and U87MG cells. These results provide evidence that Tspn8 contributes to the pathogenesis of glioblastoma probably by promoting proliferation, migration and TMZ-resistance of glioma cells. Therefore, targeting Tspn8 may provide a potential therapeutic intervention for malignant glioma. (C) 2015 Elsevier Inc. All rights reserved.
基金：
Financial disclosure: This work was supported by National
Natural Science of China (81441072), the fund of Shanghai Municipal
Health Bureau (20124324) and the fund of Shanghai Charitable
Cancer Research Center.
第一作者机构：[a]Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Ruijin Hospital affiliated to Shanghai Jiao-tong University School of Medicine, 197 Shanghai Ruijin Second Road, 200025, China
推荐引用方式(GB/T 7714)：
Si-Jian Pan,Yue-Bing Wu,Shang Cai,et al.Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,458(3):476-482.doi:10.1016/j.bbrc.2015.01.128.
APA：
Si-Jian Pan,Yue-Bing Wu,Shang Cai,Yi-Xin Pan,Wei Liu...&Qing-Fang Sun.(2015).Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,458,(3)
MLA：
Si-Jian Pan,et al."Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 458..3(2015):476-482
