机构：[1]Departments of Dental Implantology,School and Hospital of Stomatology, Jilin University,Changchun, Jilin 130021[2]Departments of Very Important People, School and Hospital of Stomatology, Jilin University,Changchun, Jilin 130021[3]Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, Jilin 130021[4]Department of Stomatology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, P.R. China口腔科首都医科大学宣武医院[5]Institute of Dental Research, Westmead Centre for Oral Health and Westmead Millennium Institute, Sydney, NSW 2145[6]Department of Oral Biology, Faculty of Dentistry, University of Sydney, Sydney, NSW 2010, Australia
Nerve injury is a serious complication associated with dental implant surgery. Following nerve injury, the migration of Schwann cells (SCs) supports nerve regeneration. Concentrated growth factor (CGF) belongs to a new generation of biomaterials that contain fibrin matrix, as well as a number of growth factors that affect the migration of various types of cells, including endothelial cells and cancer cells. To the very best of our knowledge, there are no available studies to date on the promoting effect of CGF on the migration of SCs. Thus, the aim of the present study was to characterize the structure of CGF and growth factor release, examine the effects of CGF on the migration of SCs, and to examine the role of integrin 1 and the focal adhesion kinase (FAK) pathway in the CGF-induced migration of SCs. For this purpose, CGF was prepared by centrifuging rat venous blood, which produced a fiber-like matrix capable of releasing transforming growth factor-1 (TGF-1) over a sustained period of time (at least 13 days). The soluble component of CGF was used to produce conditioned media to treat the SC cells in culture. The results demonstrated that CGF promoted the migration of SCs and increased the expression of integrin 1. These effects appeared to involve FAK phosphorylation, which occurred downstream of integrin 1 activation. The short-interfering RNA (siRNA)-mediated downregulation of integrin 1 expression did not block the ability of CGF to promote the migration of SCs. These data suggest that CGF promotes the migration of SCs partly through the integrin 1-mediated activation of the FAK pathway.
基金：
the Jilin Province Science Foundation (grant no. 2014Z044),
the Funding for Top Talents among PhD students in Jilin University,
the National Natural Science Foundation of China (Youth Scholar Program, grant no. 81400487),
the Jilin Province Science Foundation for Youths (grant no. 20150520043JH),
the Excellent Youth Scholars of Bethune Medical Department in Jilin University (grant no. 2013208064),
第一作者机构：[1]Departments of Dental Implantology,School and Hospital of Stomatology, Jilin University,Changchun, Jilin 130021[3]Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, Jilin 130021
通讯作者：
通讯机构：[*1]Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, P.R. China
推荐引用方式(GB/T 7714)：
JIE QIN,LIN WANG,LING ZHENG,et al.Concentrated growth factor promotes Schwann cell migration partly through the integrin beta 1-mediated activation of the focal adhesion kinase pathway[J].INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE.2016,37(5):1363-1370.doi:10.3892/ijmm.2016.2520.
APA：
JIE QIN,LIN WANG,LING ZHENG,XIAOYAN ZHOU,YIDI ZHANG...&YANMIN ZHOU.(2016).Concentrated growth factor promotes Schwann cell migration partly through the integrin beta 1-mediated activation of the focal adhesion kinase pathway.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,37,(5)
MLA：
JIE QIN,et al."Concentrated growth factor promotes Schwann cell migration partly through the integrin beta 1-mediated activation of the focal adhesion kinase pathway".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 37..5(2016):1363-1370
