机构：[1]General Surgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou 215004, China[2]Oncology Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou 215004, China[3]General Surgery Department, Ruijin Hospital, Shanghai Jiao Tong University, 197 Ruijin No 2 Rd, Shanghai 200025, China
To study the molecular mechanisms of colorectal cancer liver metastasis. Cecal wall implantation was performed in nude mice to subclone a highly liver metastatic human colorectal cancer clone (SW1116-M) from SW1116. In vivo and in vitro assays were adopted to confirm the proliferation and metastasis potential. The human tumor metastasis PCR microarrays were used to analyze the differential gene expressions. The results were confirmed further by real-time quantitative PCR. SW1116-M and SW1116-S5, two human colon cancer cell clones with different metastatic potential, were subcloned from SW1116. In SW1116-M, in vitro invasion, migration and in vivo metastatic potential were higher, and in vitro proliferation rate was lower than SW1116-S5. In tumor metastasis PCR microarray, 24 genes related to cell invading, adhesion, cellular growth and differentiation were found with a twofold difference between SW1116-S5 and SW1116-M. Sixteen of these, including E-cadherins, MTSS1, TRAIL and TRPM1, were up-regulated; eight genes including cathepsin L, EphB2, HGF, MET, MCAM and ROR beta were down-regulated. We have established a highly liver metastatic clone. The subsequent metastasis PCR microarray analysis identified a procedure of cellular differentiation and mesenchymal to epithelial transition (MET) in liver metastasis. The colonization to from macrometastasis is not a switch from cell cycle arrest but a result of cell differentiation and MET.
基金：
This study is sponsored by the Science, Education and Health Foundation of Soochow City (SWKQ0916).
第一作者机构：[1]General Surgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[1]General Surgery Department, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Suzhou 215004, China[3]General Surgery Department, Ruijin Hospital, Shanghai Jiao Tong University, 197 Ruijin No 2 Rd, Shanghai 200025, China
推荐引用方式(GB/T 7714)：
Qiang Chen,Lei Chen,Ren Zhao,et al.Microarray analyses reveal liver metastasis-related genes in metastatic colorectal cancer cell model[J].JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY.2013,139(7):1169-78.doi:10.1007/s00432-013-1424-2.
APA：
Qiang Chen,Lei Chen,Ren Zhao,Xiao-dong Yang,Khan Imran&Chun-gen Xing.(2013).Microarray analyses reveal liver metastasis-related genes in metastatic colorectal cancer cell model.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,139,(7)
MLA：
Qiang Chen,et al."Microarray analyses reveal liver metastasis-related genes in metastatic colorectal cancer cell model".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 139..7(2013):1169-78
