机构：[a]Departments of Neurosurgery, Stanford University, Stanford, CA 94305, United States[b]Departments of Biological Sciences, Stanford University, Stanford, CA 94305, United States[c]Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China[d]Department of Neurosurgery, Xuanwu Hospital, Capital Medical School, Beijing 100053, China神经外科首都医科大学宣武医院
Ischemic postconditioning (IPostC) protects against stroke, but few have studied the pathophysiological mechanisms of its long-term protective effects. Here, we investigated whether the mTOR pathway is involved in the long-term protective effects of IPostC. Stroke was induced in rats by distal middle cerebral artery occlusion (dMCAo) combined with 30 min of bilateral common carotid artery (CCA) occlusion, and IPostC was induced after the CCA release. Injury size and behavioral tests were measured up to 3 weeks post stroke. We used rapamycin and mTOR shRNA lentiviral vectors to inhibit mTOR activities, while S6K1 viral vectors, a main downstream mTOR gene, were used to promote mTOR activities. We found that rapamycin administration abolished the long-term protective effects of IPostC. In addition, IPostC promoted the presynaptic growth associated protein 43 (GAP-43) and the postsynaptic protein 95 (PSD-95) levels at 1 week post-stroke, which were reduced by rapamycin. Furthermore, rapamycin reduced phosphorylated mTOR (p-mTOR) protein levels measured at 3 weeks after stroke. These results were confirmed by mTOR shRNA transfection. Moreover, we found that injection of S6K1 viral vectors promoted GAP-43 and PSD-95 protein levels. We conclude that mTOR may play a crucial, protective role in brain damage after stroke and contribute to the protective effects of IPostC.
基金：
NIH/NINDS2R01NS064136 (HZ)
the National Science Foundation of China (81571111, RX).
第一作者机构：[a]Departments of Neurosurgery, Stanford University, Stanford, CA 94305, United States
共同第一作者：
通讯作者：
通讯机构：[d]Department of Neurosurgery, Xuanwu Hospital, Capital Medical School, Beijing 100053, China[*1]Department of Neurosurgery, Stanford University School of Medicine, MSLS Bldg., P306, 1201 Welch Rd., Rm. P306, Stanford, CA 94305-5327, United States.
推荐引用方式(GB/T 7714)：
Peng Wang,Rong Xie,Michelle Cheng,et al.The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke[J].NEUROSCIENCE LETTERS.2018,676:58-65.doi:10.1016/j.neulet.2018.03.062.
APA：
Peng Wang,Rong Xie,Michelle Cheng,Robert Sapolsky,Xunming Ji&Heng Zhao.(2018).The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke.NEUROSCIENCE LETTERS,676,
MLA：
Peng Wang,et al."The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke".NEUROSCIENCE LETTERS 676.(2018):58-65
