Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson's disease using nano-based microelectrode arrays
机构：[1]State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China[2]University of Chinese Academy of Sciences, Beijing 100190, China[3]Wincon TheraCells Biotechnologies Co., Ltd., Nanning 530002, China[4]Department of Neurobiology, Xuanwu Hospital, Capital Medical University, The Beijing Key Laboratory of Parkinson's Disease, Beijing 100053, China.首都医科大学宣武医院
Parkinson's disease (PD) is characterized by a progressive degeneration of nigrostriatal dopaminergic neurons. The precise mechanisms are still unknown. Since the neuronal communications are inherently electrical and chemical in nature, dual-mode detection of PD-related neuroelectrical and neurochemical information is essential for PD research. Subthalamic nucleus (STN) high-frequency stimulation (HFS) can improve most symptoms of PD patients and decrease the dosage of antiparkinsonian drugs. The mechanism of STN-HFS for PD still remains elusive. In this study, a silicon-based dual-mode microelectrode array (MEA) probe was designed and fabricated, and systematic dual-mode detection methods were established. The recording sites were modified using Pt nanoparticles and Nafion to improve the signal-to-noise (SNR) ratio. To evaluate its applicability to PD research, in vivo electrophysiological and electrochemical detection was performed in normal and hemiparkinsonian models, respectively. Through comparison of the dual-mode signals, we demonstrated the following in a PD monkey: (1) the maximum dopamine concentration in the striatum decreased by 90%; (2) the spike firing frequency increased significantly, especially in the region of the cortex; (3) the spectrogram analysis showed that much power existed in the 0-10 Hz frequency band; and (4) following repeated subthalamic nucleus high-frequency stimulation trials, the level of DA in the striatum increased by 16.5 mu M, which led to a better elucidation of the mechanism of HFS. The dual-mode MEA probe was demonstrated to be an effective tool for the study of neurological disorders.
基金：
the NSFC (Grant Nos. 61527815, 31500800, 61501426,61471342),
the National Key Research and Development Program (Grant No.2017YFA0205900),
the Beijing Science and Technology Plan (Grant Nos. Z141100000214002, Z1161100004916001),
the National Science and Technology Major Project (2014CB744600),
the Key Programs of the Chinese Academy of Sciences (Grant Nos. KJZD-EW-L11-2, QYZDJ-SSW-SYS015).
第一作者机构：[1]State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China[2]University of Chinese Academy of Sciences, Beijing 100190, China
通讯作者：
通讯机构：[1]State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China[2]University of Chinese Academy of Sciences, Beijing 100190, China[3]Wincon TheraCells Biotechnologies Co., Ltd., Nanning 530002, China[4]Department of Neurobiology, Xuanwu Hospital, Capital Medical University, The Beijing Key Laboratory of Parkinson's Disease, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Song Zhang,Yilin Song,Mixia Wang,et al.Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson's disease using nano-based microelectrode arrays[J].MICROSYSTEMS & NANOENGINEERING.2018,4:doi:10.1038/micronano.2017.70.
APA：
Song Zhang,Yilin Song,Mixia Wang,Guihua Xiao,Fei Gao...&Xinxia Cai.(2018).Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson's disease using nano-based microelectrode arrays.MICROSYSTEMS & NANOENGINEERING,4,
MLA：
Song Zhang,et al."Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson's disease using nano-based microelectrode arrays".MICROSYSTEMS & NANOENGINEERING 4.(2018)
