A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain
机构：[a]State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Science,Beijing 100190, China[b]University of Chinese Academy of Sciences,Beijing 10090, China[c]University of Kentucky Chandler Medical Center, Lexington, KY 40536–0098,USA[d]Xuanwu Hospital,Capital Medical University,Beijing 100053, China首都医科大学宣武医院
Dual-mode, multielectrode recordings have become routine in rodent neuroscience research and have recently been adapted to the non-human primate. However, robust and reliable application of acute, multielectrode recording methods in monkeys especially for deep brain nucleus research remains a challenge. In this paper, We described a low cost silicon based 16-site implantable microelectrode array (MEA) chip fabricated by standard lithography technology for in vivo test. The array was 25 mm long and designed to use in non-human primate models, for electrophysiological and electrochemical recording. We presented a detailed protocol for array fabrication, then showed that the device can record Spikes, LFPs and dopamine (DA) variation continuously from cortex to striatum in an esthetized monkey. Though our experiment, high-quality electrophysiological signals were obtained from the animal. Across any given microelectrode, spike amplitudes ranged from 70 to 300 mu V peak to peak, with a mean signal-tonoise ratio of better than 5:1. Calibration results showed the MEA probe had high sensitivity and good selectivity for DA. The DA concentration changed from 42.8 to 481.6 mu M when the MEA probe inserted from cortex into deep brain nucleus of striatum, which reflected the inhomogeneous distribution of DA in brains. Compared with existing methods allowing single mode (electrophysiology or electrochemistry) recording. This system is designed explicitly for dual-mode recording to meet the challenges of recording in non-human primates. (C) 2016 Elsevier B.V. All rights reserved.
基金：
the National Natural Science Foundation (Nos.61527815,31500800,61501426,61471342),
the Beijing Science and Technology Plan(Z141100000214002,Z141100003414015),
National Science and Technology Major Project (2014CB744600),
the Key Programs of the Chinese Academy of Sciences(No.KJZD-EW-L11–2).
第一作者机构：[a]State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Science,Beijing 100190, China[b]University of Chinese Academy of Sciences,Beijing 10090, China
通讯作者：
通讯机构：[a]State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Science,Beijing 100190, China[d]Xuanwu Hospital,Capital Medical University,Beijing 100053, China
推荐引用方式(GB/T 7714)：
Song Zhang,YilinSong,MixiaWang,et al.A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain[J].BIOSENSORS & BIOELECTRONICS.2016,85:53-61.doi:10.1016/j.bios.2016.04.087.
APA：
Song Zhang,YilinSong,MixiaWang,ZhimingZhang,XinyiFan...&XinxiaCai.(2016).A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain.BIOSENSORS & BIOELECTRONICS,85,
MLA：
Song Zhang,et al."A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain".BIOSENSORS & BIOELECTRONICS 85.(2016):53-61
工程技术