机构：[a]Department of Intensive Care Unit, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, PR China[b]Department of Biochemistry, Medical School, Nantong University, Nantong, Jiangsu, 226001, PR China[c]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing 100053, PR China首都医科大学宣武医院
The human heart expresses four isoforms of cardiac troponin T (cTnT) through alternative splicing of exons 4 and 5 of the cTnT gene. Alternative splicing of cTnT exon 5 is developmentally regulated. cTnT isoforms containing exon 5 are expressed in the fetal and neonatal heart but not in the mature heart. SRp55 is an essential splicing factor involved in cTnT exon 5 splicing and it is phosphorylated by Dyrk1A (dual specificity tyrosine phosphorylation regulated kinase 1A). In the present study, we found Dyrk1A interacted with SRp55 and enhanced its promotion of cTnT exon 5 inclusion. The shift from cTnT exon 5 inclusion to exclusion during development was delayed in the heart of Ts65Dn mice due to Dyrk1A overexpression. These results provide new insight into the role of Dyrk1A in the neonatal cardiac development. (C) 2016 Elsevier Inc. All rights reserved.
基金：
Natural Science Foundation of Nantong University (14ZY020);
National Natural Science Foundation of China Grant (grant 81503077);
Basic Research Program of Jiangsu Education Department Grant (grant13KJB320015);
a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institution(PAPD);
The Open Program of Key Laboratory Foundation of Capital Medical University (1300-1140170555).
第一作者机构：[a]Department of Intensive Care Unit, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, PR China
通讯作者：
通讯机构：[b]Department of Biochemistry, Medical School, Nantong University, Nantong, Jiangsu, 226001, PR China
推荐引用方式(GB/T 7714)：
Shu Lu,Xiaomin Yin.Overexpression of Dyrk1A regulates cardiac troponin T splicing in cells and mice[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,473(4):993-998.doi:10.1016/j.bbrc.2016.04.004.
APA：
Shu Lu&Xiaomin Yin.(2016).Overexpression of Dyrk1A regulates cardiac troponin T splicing in cells and mice.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,473,(4)
MLA：
Shu Lu,et al."Overexpression of Dyrk1A regulates cardiac troponin T splicing in cells and mice".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 473..4(2016):993-998
