机构：[1]Department of Neurology, Xuanwu Hospital, Capital Medical University, Number 45 Changchun Street, Xicheng District 100053, Beijing, China神经内科首都医科大学宣武医院[2]Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Number 15 Changle Street, Xi’an 710032, Shanxi, China
Recurrent seizures can induce mossy fiber sprouting (MFS), of the hippocampal dentate gyrus, and synaptic reorganization in mature brain. This changes local circuits and provides a structural basis for epileptogenesis in the hippocampus. However, the mechanisms of MFS and synaptic reorganization still remain unclear. Neural-cadherin (N-cadherin), a calcium adhesion molecule, plays an important role in neurite outgrowth, pathfinding, and synaptic specificity of early central nervous system development. It is unknown whether N-cadherin is involved in MFS after seizures in mature brain. To further examine the correlation between MFS and N-cadherin expression, we separately labeled MFS and N-cadherin with Timm staining and antibody in adult rats after status epilepticus (SE). Timm staining revealed that MFS is observed in the inner molecular layer of dentate gyrus of rats 2 and 4 weeks after SE. The observed MFS migrated from the hilus to the granule cell layer, gradually extending axons into the inner molecular layer to form an intense band. Immunohistochemical staining of N-cadherin revealed that the upregulated expression of N-cadherin was concentrated in the position of mossy fiber axonal sprouts of rats 1-4 weeks after SE, and that it was earlier than MFS. The spatial and temporal distribution consistence of N-cadherin and Timm staining supported the correlation that exists between N-cadherin expression and the process of aberrant MFS. This result suggests that N-cadherin may be involved in the pathfinding and synaptic specificity of MFS in mature brain after seizures, and can play an important role in the targeted growth of mossy fibers.
基金：
Neurobiology Department and Experimental Animal Center of the Fourth Military Medical University.
第一作者机构：[1]Department of Neurology, Xuanwu Hospital, Capital Medical University, Number 45 Changchun Street, Xicheng District 100053, Beijing, China
通讯作者：
通讯机构：[1]Department of Neurology, Xuanwu Hospital, Capital Medical University, Number 45 Changchun Street, Xicheng District 100053, Beijing, China
推荐引用方式(GB/T 7714)：
Hua Lin,Yuangui Huang,Yuping Wang,et al.Spatiotemporal profile of N-cadherin expression in the mossy fiber sprouting and synaptic plasticity following seizures[J].MOLECULAR AND CELLULAR BIOCHEMISTRY.2011,358(1-2):201-205.doi:10.1007/s11010-011-0935-x.
APA：
Hua Lin,Yuangui Huang,Yuping Wang&Jianping Jia.(2011).Spatiotemporal profile of N-cadherin expression in the mossy fiber sprouting and synaptic plasticity following seizures.MOLECULAR AND CELLULAR BIOCHEMISTRY,358,(1-2)
MLA：
Hua Lin,et al."Spatiotemporal profile of N-cadherin expression in the mossy fiber sprouting and synaptic plasticity following seizures".MOLECULAR AND CELLULAR BIOCHEMISTRY 358..1-2(2011):201-205
