机构：[a]Center of Clinical Pathology, China Capital Medical University, Beijing, China[b]Department of Pathology, Xuanwu Hospital of China Capital Medical University, Beijing, China病理科首都医科大学宣武医院[c]Department of Pathology, Beijing Tongren affiliated Hospital of China Capital Medical University, Beijing, China
Aims: Anthracosis is the deposition of black dusty material in the pulmonary parenchyma. Previous reports showed anthracosis and p16(ink4a) gene aberrant methylation are closely related to the promotion and progression of small-sized pulmonary adenocarcinoma. In this study, we investigated the influence of anthracosis and p16(ink4a) gene aberrant methylation on clinical samples from patients with small-sized adenocarcinoma. Methods and results: DNA was bisulfite modified and methylation-specific PCR was performed to detect p16(ink4a) gene aberrant methylation; black dusty material was extracted from lung tissues. Anthracotic index (AI) was defined as the absolute absorbance by densitometry. The histopathological diagnosis was concluded according to Noguchi's classification for small-sized pulmonary adenocarcinoma. The mean AI and the frequency of p16(ink4a) gene aberrant methylation of heavy smokers were significantly higher than that of nonsmokers ( <0.01 and <0.05, respectively). The frequency of p16(ink4a) gene aberrant methylation of early stage small-sized adenocarcinoma was lower than that of advanced and poorly differentiated, while p16(ink4a) protein expression level of early stage small-sized adenocarcinoma was significantly higher than that of poorly differentiated small-sized adenocarcinoma (P<0.05). Conclusions: AI and p16(ink4a) gene aberrant methylation may provide a potential universal biomarker for small-sized adenocarcinoma. (C) 2010 Elsevier Inc. All rights reserved.
第一作者机构：[a]Center of Clinical Pathology, China Capital Medical University, Beijing, China[*]Center of Clinical Pathology, China Capital Medical University, No.10 Xitoutiao, You An Men street, Beijing 100069, P.R. China.
通讯作者：
通讯机构：[*]Center of Clinical Pathology, China Capital Medical University, No.10 Xitoutiao, You An Men street, Beijing 100069, P.R. China.
推荐引用方式(GB/T 7714)：
Daye Wang,Jue Wang,Yong Li,et al.The influence of anthracosis and p16(ink4a) gene aberrant methylation on small-sized pulmonary adenocarcinoma[J].EXPERIMENTAL AND MOLECULAR PATHOLOGY.2011,90(1):131-136.doi:10.1016/j.yexmp.2010.10.014.
APA：
Daye Wang,Jue Wang,Yong Li,Zhili He&Yong Zhang.(2011).The influence of anthracosis and p16(ink4a) gene aberrant methylation on small-sized pulmonary adenocarcinoma.EXPERIMENTAL AND MOLECULAR PATHOLOGY,90,(1)
MLA：
Daye Wang,et al."The influence of anthracosis and p16(ink4a) gene aberrant methylation on small-sized pulmonary adenocarcinoma".EXPERIMENTAL AND MOLECULAR PATHOLOGY 90..1(2011):131-136
