机构：[a]Department of Neurology and Neurobiology, College of Biological Sciences, Biological Sciences Center, University of Minnesota, St. Paul, Minnesota, USA[b]The Rhythmometry Laboratory, College of Biological Sciences, Biological Sciences Center, University of Minnesota, St. Paul, Minnesota, USA[c]Cell Therapy Center, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing P. R. China神经变性病教育部重点实验室首都医科大学宣武医院[d]Laboratory of Cell and Development, College of Life Sciences, Capital Normal University, Beijing P. R. China
E-boxes targeted by BMAL1-CLOCK dimers are crucial for the rhythmic expression of mPer1. We investigated whether DNA methylation, especially E-box CpG methylation, plays a role in the regulation of mPer1 oscillations in mice. E-box-containing amplicons were examined in liver, thymus and testis around-the-clock using bisulfite sequencing, combined bisulfite restriction analysis (COBRA) and bisulfite pyrosequencing. Results indicated that mPer1 E-boxes were only hypomethylated in all tissues at all circadian stages, suggesting that E-boxes are open to BMAL1-CLOCK dimers at all times, and thus E-box methylation is most likely not involved in global regulation of the mPer1 rhythm. In addition, low but noticeable methylation of E-box 3 and/or 4 and a sub-group of CpGs nearby were identified in all three tissues, indicating this region as area of preferred methylation, and may have a role in silencing mPer1 transcription.
基金：
National Natural Science Foundation of China (30400148, 30430280); The National Basic Research Program of China (2006CB500701, 2006CB943703);
The National 863 Project grant of China (2006AA02A408); Scientific project of Beijing municipal science and technology commission (D07050701350703);
The Natural Sciences Foundation of Beijing (5063042); and New Century Excellent Talents in University (2007),
and for RBS, in part, by financial support from Jane and Mike Popovich
第一作者机构：[a]Department of Neurology and Neurobiology, College of Biological Sciences, Biological Sciences Center, University of Minnesota, St. Paul, Minnesota, USA
共同第一作者：
通讯作者：
通讯机构：[a]Department of Neurology and Neurobiology, College of Biological Sciences, Biological Sciences Center, University of Minnesota, St. Paul, Minnesota, USA
推荐引用方式(GB/T 7714)：
Yanning Cai,Shu Liu,Robert B. Sothern,et al.Epigenetic analysis of mPer1 promoter in peripheral tissues[J].BIOLOGICAL RHYTHM RESEARCH.2009,40(6):445-453.doi:10.1080/09291010802568822.
APA：
Yanning Cai,Shu Liu,Robert B. Sothern,Ning Li,Yunqian Guan&Piu Chan.(2009).Epigenetic analysis of mPer1 promoter in peripheral tissues.BIOLOGICAL RHYTHM RESEARCH,40,(6)
MLA：
Yanning Cai,et al."Epigenetic analysis of mPer1 promoter in peripheral tissues".BIOLOGICAL RHYTHM RESEARCH 40..6(2009):445-453
