机构：[1]College of Food Science & Nutritional Engineering, China Agricultural University Beijing 100083, China,[2]Saisheng Pharmaceutical Company, Beijing 100176, China,[3]Department of Neurology XuanWU Hospital, Capital University of Medical Sciences, Beijing 100069, China神经内科首都医科大学宣武医院
To purify and characterize the fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Monoclonal antibodies against fibrinogenase were prepared and a novel affinity chromatography equipped with a monoclonal antibody against fibrinogenase was developed and applied for the purification of fibrinogenases. The purified fibrinogenase was identified by fibrinolytic activity assay, and antithrombosis activity assay. HPLC chromatography and SDS-PAGE analysis demonstrated the uniformity and purity of the purified fibrinogenase. In comparison with a conventional A-50 chromatography method, affinity-purified fibrinogenase showed higher activity (3631 U mg(-1) vs 501 U mg(-1)). In addition, the physiological activity of the fibrinogenase both in vitro and ex vivo showed the purified fibrinogenase can specifically degrade beta-, gamma-fibrinogen and has a high anti-thrombotic activity. In conclusion, the purified fibrinogenase by affinity column were shown to be homogeneous and showed a high and specific proteolytic activity against beta-chains of fibrinogen molecules and antithrombosis activity.
第一作者机构：[1]College of Food Science & Nutritional Engineering, China Agricultural University Beijing 100083, China,[2]Saisheng Pharmaceutical Company, Beijing 100176, China,
通讯作者：
通讯机构：[1]College of Food Science & Nutritional Engineering, China Agricultural University Beijing 100083, China,
推荐引用方式(GB/T 7714)：
Biao Ma,Ying Zhang,Dan Wu,et al.Affinity-purification of fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom[J].ARCHIVES OF PHARMACAL RESEARCH.2008,31(9):1129-1136.doi:10.1007/s12272-001-1279-1.
APA：
Biao Ma,Ying Zhang,Dan Wu,Jianping Jia,Wentao Xu&Yunbo Luo.(2008).Affinity-purification of fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom.ARCHIVES OF PHARMACAL RESEARCH,31,(9)
MLA：
Biao Ma,et al."Affinity-purification of fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom".ARCHIVES OF PHARMACAL RESEARCH 31..9(2008):1129-1136
