机构：[a]Neurosurgery Department, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, China[b]Department of Ultrasound, The Second Affiliated Hospital of Soochow University, Suzhou, China
Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) overexpression promotes glioma cell progression. The aim of the current study is to silence IGF2BP1 in glioma cells by the microRNA (miRNA) strategy. The bio-informatic analyses identified that microRNA-4500 (miR-4500) putatively targets 3′-UTR (3′-untranslated region) of IGF2BP1. In A172 cells and primary human glioma cells ectopic overexpression of the wild-type miR-4500 (but not the mutant form) downregulated IGF2BP1 and its target genes (Gli1, IGF2 and c-Myc). Functional studies show that ectopic miR-4500 overexpression inhibited glioma cell growth, survival, proliferation, migration and invasion. Conversely, in A172 cells miR-4500 inhibition, by a lentiviral construct, increased expression of IGF2BP1 and its targets, promoting cell survival, proliferation and migration. Furthermore, IGF2BP1 knockout by the CRISPR/Cas9 method inhibited A172 cell progression. Significantly, miR-4500 overexpression or miR-4500 inhibition was ineffective in IGF2BP1 knockout A172 cells. At last, we show that miR-4500 levels are downregulated in human glioma tissues, correlating with IGF2BP1 upregulation. Together, we conclude that miR-4500 inhibits human glioma cell progression by targeting IGF2BP1. ? 2019 Elsevier Inc.
基金：
This work was generously supported by the Fundings from Xuzhou Medical University .
第一作者机构：[a]Neurosurgery Department, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, China
共同第一作者：
通讯作者：
通讯机构：[*1]Neurosurgery Department, Xuzhou Children's Hospital, Xuzhou Medical University, No. 18 Sudi North Road, Quanshan District, Xuzhou, China.
推荐引用方式(GB/T 7714)：
Zheng-wei Li,Min Xue,Bing-Xin Zhu,et al.microRNA-4500 inhibits human glioma cell progression by targeting IGF2BP1[J].Biochemical and Biophysical Research Communications.2019,513(4):800-806.doi:10.1016/j.bbrc.2019.04.058.
APA：
Zheng-wei Li,Min Xue,Bing-Xin Zhu,Cheng-long Yue,Min Chen&Hai-hui Qin.(2019).microRNA-4500 inhibits human glioma cell progression by targeting IGF2BP1.Biochemical and Biophysical Research Communications,513,(4)
MLA：
Zheng-wei Li,et al."microRNA-4500 inhibits human glioma cell progression by targeting IGF2BP1".Biochemical and Biophysical Research Communications 513..4(2019):800-806
