机构：[a]Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China[b]Department of Anesthesiology, Xuanwu Hospital Capital Medical University, Beijing, China麻醉手术科[c]Department of Anesthesiology, The First Affiliated Hospital Sun Yat-sen University, Guangzhou, China[d]Department of Anesthesiology, Tianjin Medical University Cancer Hospital, Tianjin, China[e]Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China[f]Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, China[g]Department of Critical Care Medicine, The first Affiliated Hospital of Harbin Medical University, China
Colorectal cancer (CRC) is the third most common cancer worldwide. To date, no non-invasive and specific biomarkers have been identified for the diagnosis of CRC. The analysis of volatile organic compounds (VOCs) is attracting increasing attention and provides the possibility of a non-invasive diagnosis. Solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) have been used to analyze the VOCs released from the headspace gas of LS174T (Dukes' type B colorectal adenocarcinoma) cells, arsenic trioxide (ATO)-treated LS174T cells and the blood from tumor-bearing mice. The data were processed using principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA), which showed that the levels of decanal, 2,4-dimethyl- heptane, and twelve other metabolites were significantly greater in the headspace gas of the LS174T cells and blood of tumor-bearing mice. Additionally, in vivo experiments indicated that formic acid, ethenyl ester and p-trimethylsilyloxyphenyl-(trimethylsilyloxy)trimethylsilylacrylate were consumed during tumor growth. In conclusion, VOCs such as 1-methoxy-hexane and 2,4-dimethyl-heptane could be useful diagnostic markers for CRC. Further research should focus on the potential metabolic pathways associated with these profiles.
Copyright ? 2019. Published by Elsevier Inc.
基金：
国家自然科学基金
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区分析化学4 区生化研究方法4 区生化与分子生物学
最新[2023]版：
大类|4 区生物学
小类|3 区分析化学4 区生化研究方法4 区生化与分子生物学
JCR分区：
出版当年[2017]版：
Q2CHEMISTRY, ANALYTICALQ3BIOCHEMISTRY & MOLECULAR BIOLOGYQ3BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICALQ3BIOCHEMISTRY & MOLECULAR BIOLOGY
第一作者机构：[a]Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China[b]Department of Anesthesiology, Xuanwu Hospital Capital Medical University, Beijing, China
通讯作者：
通讯机构：[a]Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China[g]Department of Critical Care Medicine, The first Affiliated Hospital of Harbin Medical University, China
推荐引用方式(GB/T 7714)：
Miao Liu,Yuhang Li,Guiyue Wang,et al.Release of volatile organic compounds (VOCs) from colorectal cancer cell line LS174T.[J].Analytical biochemistry.2019,581:113340.doi:10.1016/j.ab.2019.06.011.
APA：
Miao Liu,Yuhang Li,Guiyue Wang,Nana Guo,Desheng Liu...&Changsong Wang.(2019).Release of volatile organic compounds (VOCs) from colorectal cancer cell line LS174T..Analytical biochemistry,581,
MLA：
Miao Liu,et al."Release of volatile organic compounds (VOCs) from colorectal cancer cell line LS174T.".Analytical biochemistry 581.(2019):113340
