Short-Chain Fatty Acids (SCFAs) represent the largest group of metabolic nutrients produced by gut microbiota, and can be considered potential biomarkers for digestive tract diseases. A fast, cost-effective, and reproducible analytical method for the analysis of SCFAs is highly required. This work developed a one-step extraction of 14 SCFAs using a mixture of ethanol and n-hexane in human serum samples by GC-MS without derivatization. The recoveries of the 14 SCFAs ranged from 62.4% to 129.2% in human serum samples. The LODs and LOQs of the 14 SCFAs were 0.12-0.48 mg/L and 0.40-1.61 mg/L, respectively. The application of this method was carried out by comparing SCFAs in serum samples from children with health and digestive tract diseases, and the results demonstrated that SCFAs in the serum of children with digestive disorders were significantly higher than those of healthy children, implying that digestive tract diseases affect gut microbiota and their metabolisms. [GRAPHICS] .
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81803214]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区化学
小类|4 区生化研究方法4 区分析化学
最新[2023]版：
大类|4 区化学
小类|4 区生化研究方法4 区分析化学
JCR分区：
出版当年[2017]版：
Q3CHEMISTRY, ANALYTICALQ4BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q4BIOCHEMICAL RESEARCH METHODSQ4CHEMISTRY, ANALYTICAL
第一作者机构：[1]Capital Med Univ, Natl Ctr Childrens Hlth, Beijing Childrens Hosp, Lab Nutr & Dev,Beijing Pediat Res Inst, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Natl Ctr Childrens Hlth, Beijing Childrens Hosp, Lab Nutr & Dev,Beijing Pediat Res Inst, Beijing 100045, Peoples R China;[2]China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China;[3]Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Rui,Fan Chaonan,Fan Xiuqin,et al.A Fast and Accurate Way to Determine Short Chain Fatty Acids in Human Serum by GC-MS and Their Distribution in Children with Digestive Diseases[J].CHROMATOGRAPHIA.2019,-.doi:10.1007/s10337-019-03831-9.
APA：
Wang, Rui,Fan, Chaonan,Fan, Xiuqin,Zhao, Yunfeng,Wang, Yuanyuan...&Qi, Kemin.(2019).A Fast and Accurate Way to Determine Short Chain Fatty Acids in Human Serum by GC-MS and Their Distribution in Children with Digestive Diseases.CHROMATOGRAPHIA,,
MLA：
Wang, Rui,et al."A Fast and Accurate Way to Determine Short Chain Fatty Acids in Human Serum by GC-MS and Their Distribution in Children with Digestive Diseases".CHROMATOGRAPHIA .(2019):-
