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Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect Neisseria meningitidis

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资源类型：

收录情况： ◇ SCIE

作者：
Shijun Li[1,*2] * ; Chunting Liu[1] * ; Ying Liu[1] ; Qing Ma[1] ; Yue Wang[1] ; Yi Wang[2,3,*1] ;
机构： [1]Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, 550004, People’s Republic of China [2]Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, 10045, People’s Republic of China [3]Ministry of Education, National Key Discipline of Pediatrics (Capital Medial University), Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, 10045, People’s Republic of China
出处：
DOI： 10.2147/IDR.S210735
ISSN：

关键词： Neisseria meningitidis multiple cross displacement amplification lateral flow biosensor MCDA-LFB limit of detection

摘要：
Background: Neisseria meningitidis is a leading pathogen of meningococcal disease in humans worldwide. Multiple cross displacement mplification (MCDA) combined with nanoparticles-based lateral flow biosensor (MCDA-LFB) has been reported for the rapid detection of several bacterial pathogens in recent years. Here, therefore we developed an MCDA-LFB assay for the rapid detection of N. meningitis. Methods: A set of 10 primers specifically to recognize 10 different regions of the ctrA gene of N. meningitidis were designed. MCDA was developed and combined with a LFB to detect the ctrA gene of N. meningitidis. The reaction time and temperature condition for the MCDA-LFB were optimized and then the MCDA-LFB was applied to detect the DNA from clinical samples. Results: MCDA-LFB assay was successfully established for the detection of N. meningitidis based on the ctrA gene. The MCDA assay was optimized at 64 degrees C for only 35 mins and the products of amplification were directly sensed by LFB. The whole operation, including DNA template preparation (similar to 20 mins), MCDA reaction (35 mins) and results interpretation (similar to 2 mins) could be finished in no more than 60 mins. The detection limit was as low as 10 fg/reaction (around 3 CFUs/reaction) of pure N. meningitidis DNA, with no cross-reaction with other bacterial DNA. Conclusion: The MCDA-LFB techniques developed in the present study are an effective tool for the rapid detection of N. meningitidis, especially in resource-poor countries in meningococcal disease epidemic period.

基金：
Special Funds of Program of Scientific and Technological Innovation Team of Guizhou Province [Qian Ke He Platform talent [2018]5606]; Special Funds for High-Level Creative Talents Cultivation in Guizhou Province [Qian Ke He (2016)4021]; Special Funds for the Cultivation of Outstanding Youth Talents of Science and Technology in Guizhou Province [Qian Ke He Ren Word [2015] 09]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类 | 2 区 医学
小类 | 2 区 药学 3 区 传染病学
最新[2023]版：
大类 | 3 区 医学
小类 | 3 区 传染病学 3 区 药学
JCR分区：
出版当年[2017]版：
Q2 INFECTIOUS DISEASES Q2 PHARMACOLOGY & PHARMACY
最新[2023]版：
Q2 INFECTIOUS DISEASES Q2 PHARMACOLOGY & PHARMACY

影响因子： 最新[2023版] 最新五年平均 出版当年[2017版] 出版当年五年平均 出版前一年[2016版] 出版后一年[2018版]

第一作者：
第一作者机构： [1]Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, 550004, People’s Republic of China [*2]Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou 550004, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构： [1]Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, 550004, People’s Republic of China [2]Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, 10045, People’s Republic of China [3]Ministry of Education, National Key Discipline of Pediatrics (Capital Medial University), Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, 10045, People’s Republic of China [*1]Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing 10045, China [*2]Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou 550004, People’s Republic of China
推荐引用方式(GB/T 7714)：
Shijun Li,Chunting Liu,Ying Liu,et al.Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect Neisseria meningitidis[J].INFECTION AND DRUG RESISTANCE.2019,12:2077-2087.doi:10.2147/IDR.S210735.
APA：
Shijun Li,Chunting Liu,Ying Liu,Qing Ma,Yue Wang&Yi Wang.(2019).Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect Neisseria meningitidis.INFECTION AND DRUG RESISTANCE,12,
MLA：
Shijun Li,et al."Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect Neisseria meningitidis".INFECTION AND DRUG RESISTANCE 12.(2019):2077-2087

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