Low frequency, weak MCP-1 secretion and exhausted immune status of peripheral monocytes were associated with progression of severe enterovirus A71-infected hand, foot and mouth disease
机构：[1]Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing,[2]National Clinical Key Department of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, Beijing,[3]Center of Laboratory Medicine, Beijing Children Hospital, Beijing, China职能科室临床流行病与循证医学中心首都医科大学附属北京儿童医院
A minority of hand, foot and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) results in severe neural complications. However, whether monocyte-mediated immunity is involved in the disease progression of HFMD remains unknown. One hundred and twenty mild and 103 severe HFMD patients were recruited and enzyme-linked immunosorbent assay (ELISA), flow cytometry and Transwell culture were performed in the study. Peripheral monocyte counts were lower in both absolute counts and frequencies in severe cases compared to mild cases. After screening 10 monocyte-related cytokines by ELISA, only monocyte chemoattractant protein-1 (MCP-1) was found at higher levels in sera of mild cases compared to those with severe symptoms. Monocytes purified from mild cases produced more MCP-1 than the cells from severe patients when stimulated in vitro. We observed that immune exhaustion markers programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) were highly regulated on the surface of monocytes from severe cases compared to mild cases. PD-L1 blockade induced a higher production of MCP-1 in the supernatant of a Transwell system. The production of MCP-1 also increased following PD-L1 blockade of purified monocytes activated by granulocyte-macrophage colony-stimulating factor (GM-CSF) combined with R848 or EV-A71 virus. Our results indicate that absolute count, frequency and levels of MCP-1 secretion of peripheral monocytes, together with their immune status, probably contribute to differential disease prognosis in EV-A71-associated HFMD.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81772184]
第一作者机构：[1]Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing,[*1]Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences Peking University, Beijing 100083, China.[*2]Department of Microbiology and Infectious Disease Center, Peking University Health Science Center, Room 329, Building of Pathology, 38# Xueyuan Rd, Beijing, 100083, China.
推荐引用方式(GB/T 7714)：
Pei X.,Fan X.,Zhang H.,et al.Low frequency, weak MCP-1 secretion and exhausted immune status of peripheral monocytes were associated with progression of severe enterovirus A71-infected hand, foot and mouth disease[J].CLINICAL AND EXPERIMENTAL IMMUNOLOGY.2019,196(3):353-363.doi:10.1111/cei.13267.
APA：
Pei, X.,Fan, X.,Zhang, H.,Duan, H.,Xu, C....&Shen, T..(2019).Low frequency, weak MCP-1 secretion and exhausted immune status of peripheral monocytes were associated with progression of severe enterovirus A71-infected hand, foot and mouth disease.CLINICAL AND EXPERIMENTAL IMMUNOLOGY,196,(3)
MLA：
Pei, X.,et al."Low frequency, weak MCP-1 secretion and exhausted immune status of peripheral monocytes were associated with progression of severe enterovirus A71-infected hand, foot and mouth disease".CLINICAL AND EXPERIMENTAL IMMUNOLOGY 196..3(2019):353-363
