机构：[1]College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, Baoding 071002, China[2]Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China[3]University of Chinese Academy of Sciences, Beijing 100049, China[4]National Center for Children’s Health, Beijing Children’s Hospital, Beijing 100045, China职能科室临床流行病与循证医学中心首都医科大学附属北京儿童医院
A new open-tubular capillary electrochromatography (OT-CEC) method for analysis of beta-lactam antibiotics has been developed with unique block co-polymer coating. To obtain the highly ordered block polymer chains, reversible addition fragmentation chain transfer radical polymerization method was used to synthesize poly(maleic anhydride-styrene-N-isopropylacrylamide). The prepared block copolymer coating was characterized with NMR, Fourier transform infrared spectroscopy and scanning electron microscope. Several key separation factors of OT-CEC, which including polymer amount, stability of the coating, temperature, species of organic additives, buffer pH and concentration, were investigated in detail. Our results indicated that the separation efficiency was improved greatly with the coating capillary and the three test analytes could be baseline separated. Then, the separation mechanism was briefly explored. Moreover, the proposed OT-CEC method displayed promising quantitative analysis property of the three test analytes with good linearity (R-2 > 0.99), repeatability (relative standard deviations <0.9%) and high recovery (95.4%-106.2%). Further, the assay was applied in monitoring the three test beta-lactam antibiotics (cephradine, cephalexin and amoxicillin) in serum samples, providing a useful platform for construction of novel polymer coatings in OT-CEC system and for analysis of drugs in real bio-samples. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21727809, 21635008, 21621062]; Chinese Academy of SciencesChinese Academy of Sciences [QYZDJ-SSW-SLH034]
第一作者机构：[1]College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, Baoding 071002, China[2]Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
通讯作者：
通讯机构：[1]College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, Baoding 071002, China[2]Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China[3]University of Chinese Academy of Sciences, Beijing 100049, China[*1]Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
推荐引用方式(GB/T 7714)：
Zhao Liping,Qiao Juan,Zhang Hongyi,et al.Open tubular capillary electrochromatography with block co-polymer coating for separation of beta-lactam antibiotics[J].CHINESE CHEMICAL LETTERS.2019,30(2):349-352.doi:10.1016/j.cclet.2018.03.007.
APA：
Zhao, Liping,Qiao, Juan,Zhang, Hongyi,Xie, Fangnan&Qi, Li.(2019).Open tubular capillary electrochromatography with block co-polymer coating for separation of beta-lactam antibiotics.CHINESE CHEMICAL LETTERS,30,(2)
MLA：
Zhao, Liping,et al."Open tubular capillary electrochromatography with block co-polymer coating for separation of beta-lactam antibiotics".CHINESE CHEMICAL LETTERS 30..2(2019):349-352
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