Recently, more and more researchers have focused on airway stent applied in tracheomalacia. The airway stents for clinical application were usually manufactured in accordance with a fixed pattern, which were difficult to perfect match with children, especially infants. Digital light procession of light curing acrylate resin implantation showed higher accuracy and printing speed over traditional three-dimensional printing techniques. In this article, a novel personalized airway stent was developed by digital light procession three-dimensional printing and was modified by collagen I extracted from the fish scales. The morphology of the collagen-modified airway stent was examined by scanning electron microscopy, and the chemical structures were examined by attenuated total internal reflectance Fourier transform infrared spectroscopy. The biocompatibility of this synthetic acrylate/collagen composite airway stent was characterized by water contact angle test and cell culture. The results confirmed that the composite airway stent was hydrophilic and non-cytotoxic toward a cultured human bronchial epithelial cell line with good cell viability and show excellent physicochemical and biological properties. In conclusion, this study presented the three-dimensional printing composite acrylate and collagen airway stent may have potential in customized treatment for tracheomalacia.