N-methyl-D-aspartate receptor expression in the spinal dorsal horn of a rat model of formalin-induced inflammatory pain following intrathecal injection of butorphanol
Clinical and animal experiments have proved that intrathecal injection of butorphanol has an analgesic effect. However, whether the analgesic effect is associated with activation of the N-methyl-D-aspartate (NMDA) receptor remains unclear. This study presumed that intrathecal injection of butorphanol has an analgesic effect on formalin-induced inflammatory pain in rats, and its analgesic effect is associated with inhibition of NMDA receptors. Concurrently, ketamine was injected into the intrathecal space, which is a non-competitive NMDA receptor antagonist, to determine the analgesic mechanism of butorphanol. The total reflection time in phase 1 and phase 2 of rat hind paws carding action was reduced when the butorphanol dose was increased to 25 mu g, or a low dose of butorphanol was combined with ketamine. Intrathecal injection of a high dose of butorphanol alone or a low dose of butorphanol combined with ketamine can remarkably reduce NMDA receptor expression in the L-5 spinal dorsal horn of formalin-induced pain rats. The results suggest that intrathecal injection of butorphanol has analgesic effects on formalin-induced inflammatory pain, and remarkably reduces NMDA receptor expression in the rat spinal dorsal horn. Ketamine strengthens this analgesic effect. The analgesic mechanism of intrathecal injection of butorphanol is associated with inhibition of NMDA receptor activation.
基金：
Department of Science and Technology of Hunan Province [2009SK3112]; Department of Health of Hunan Province [C2008005]
通讯机构：[1]Cent S Univ, Dept Anesthesiol, Xiangya Hosp, Changsha 410006, Hunan, Peoples R China;
推荐引用方式(GB/T 7714)：
Wang Yichun,Zhang Yuan,Guo Qulian,et al.N-methyl-D-aspartate receptor expression in the spinal dorsal horn of a rat model of formalin-induced inflammatory pain following intrathecal injection of butorphanol[J].NEURAL REGENERATION RESEARCH.2010,5(21):1617-1622.doi:10.3969/j.issn.1673-5374.2010.21.003.
APA：
Wang, Yichun,Zhang, Yuan,Guo, Qulian,Liu, Xiaohong,Wang, Mingde&Luo, Hui.(2010).N-methyl-D-aspartate receptor expression in the spinal dorsal horn of a rat model of formalin-induced inflammatory pain following intrathecal injection of butorphanol.NEURAL REGENERATION RESEARCH,5,(21)
MLA：
Wang, Yichun,et al."N-methyl-D-aspartate receptor expression in the spinal dorsal horn of a rat model of formalin-induced inflammatory pain following intrathecal injection of butorphanol".NEURAL REGENERATION RESEARCH 5..21(2010):1617-1622
