A collection of 45 epidemiologically unrelated Streptococcus agalactiae strains (group B Streptococcus, GBS), belonging to different serotypes, isolated from pregnant women in China and Russia was studied. Strains were characterized by pulsed-field gel electrophoresis (PFGE) employing hybridization with nine genes potentially involved in virulence. Molecular sizes of GBS genomes varied from 2030 to 2290 kb. Location of the genes under study bac, bca, glnA, scpB, cyl, hylB, lmb, scaA and cfb on the GBS genomes was found to be conserved irrelevant to the serotype. Potential virulence genes scpB, hylB, lmb were located on a 91-kb SmaI fragment that is equal to 4.5% of total genome. Ribotyping of the strains under study revealed three different HindIII, nine EcoRI and 12 PvuII ribotypes among 45 strains. A strong correlation between the PvuII ribotype and the presence of the bac gene was observed, with 21 of 22 bac-positive strains belonging to the same Pvull ribotype P1. PFGE patterns of bac-positive strains were also similar. The possibility of close genetic relatedness of all bac-positive strains is discussed. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
基金：
FIC NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Fogarty International Center (FIC) [TW00188]; NIAID NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI19304]
第一作者机构：[1]Capital Univ Med Sci, Beijing Childrens Hosp, Beijing 100045, Peoples R China;[2]Russian Acad Med Sci, Inst Expt Med, St Petersburg 197376, Russia;[3]Capital Univ Med Sci, Beijing Childrens Hosp, Nan Lishi Rd, Beijing 100045, Peoples R China
通讯作者：
通讯机构：[1]Capital Univ Med Sci, Beijing Childrens Hosp, Beijing 100045, Peoples R China;[2]Russian Acad Med Sci, Inst Expt Med, St Petersburg 197376, Russia;[3]Capital Univ Med Sci, Beijing Childrens Hosp, Nan Lishi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Dmitriev A,Hu YY,Shen AD,et al.Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous[J].FEMS MICROBIOLOGY LETTERS.2002,208(1):93-98.doi:10.1111/j.1574-6968.2002.tb11066.x.
APA：
Dmitriev, A,Hu, YY,Shen, AD,Suvorov, A&Yang, YH.(2002).Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous.FEMS MICROBIOLOGY LETTERS,208,(1)
MLA：
Dmitriev, A,et al."Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous".FEMS MICROBIOLOGY LETTERS 208..1(2002):93-98
