Inhibitory effects of C-type natriuretic peptide on the differentiation of cardiac fibroblasts, and secretion of monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1
The present study aimed to investigate the effect of C-type natriuretic peptide (CNP) on the function of cardiac fibroblasts (CFs). Western blotting was used to investigate the expression of myofibroblast marker proteins: -smooth muscle actin (-SMA), extra domain-A fibronectin, collagen I and collagen III, and the activity of extracellular signal-regulated kinase 1/2 (ERK1/2). Immunofluorescence was used to examine the morphological changes; a transwell assay was used to analyze migration, and reverse transcription-quantitative polymerase chain reaction and ELISA were employed to determine the mRNA expression and protein secretion of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1). The results demonstrated that CNP significantly reduced the protein expression of -SMA, fibronectin, collagen I and collagen III, and suppressed the migratory ability of CFs. Additionally, the mRNA and protein expression of MCP-1 and PAI-1 was inhibited under the CNP treatment; and this effect was mediated by the inhibition of the ERK1/2 activity. In conclusion, CNP inhibited cardiac fibroblast differentiation and migration, and reduced the secretion of MCP-1 and PAI-1, which demonstrates novel mechanisms to explain the antifibrotic effect of CNP.
第一作者机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Dept Pediat, Cardiac Surg Ctr, Beijing 100029, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Dept Pediat, Cardiac Surg Ctr, Beijing 100029, Peoples R China;[2]Capital Med Univ, Beijing Anzhen Hosp, Pediat Heart Ctr, 2 Anzhen Rd, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714)：
Li Zhi-Qiang,Liu Ying-Long,Li Gang,et al.Inhibitory effects of C-type natriuretic peptide on the differentiation of cardiac fibroblasts, and secretion of monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1[J].MOLECULAR MEDICINE REPORTS.2015,11(1):159-165.doi:10.3892/mmr.2014.2763.
APA：
Li, Zhi-Qiang,Liu, Ying-Long,Li, Gang,Li, Bin,Liu, Yang...&Liu, Ai-Jun.(2015).Inhibitory effects of C-type natriuretic peptide on the differentiation of cardiac fibroblasts, and secretion of monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1.MOLECULAR MEDICINE REPORTS,11,(1)
MLA：
Li, Zhi-Qiang,et al."Inhibitory effects of C-type natriuretic peptide on the differentiation of cardiac fibroblasts, and secretion of monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1".MOLECULAR MEDICINE REPORTS 11..1(2015):159-165
