Aim: To explore the influence of different hands' response on the conflict monitoring event-related potential N270. Methods: Subjects were asked to discriminate whether the two sequentially presented visual stimuli were of the same color while event-related potentials were recorded from their scalps. In the first half of the test, subjects used two different fingers of one hand to press the buttons of a pushpad, while they used the other hand in the other half. Results: N270 component was recorded only in the conflict condition mainly on the centra-frontal area. The mean amplitude of contingent negative variation (CNV) was significantly higher to the right hand response, and the lasting effect of it did not affect the amplitudes of N160, N200 and N270.The mean amplitudes of N160 and N200 were significantly higher in the left hand response. However, the mean amplitudes of N270 showed no significant difference between left and right hand responses. Conclusion: Different hands' response didn't affect the conflict monitoring event-related potential N270.