机构：[1]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Laboratory of Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou, China[2]The Second Affiliated Hospital of Soochow University, Suzhou, China[3]Chengdu Radio and TV University, Chengdu, China
AimsInsular cortex (IC) is involved in processing the information of pain. The aim of this study was to investigate roles and mechanisms of P2X7 receptors (P2X7Rs) in IC in development of visceral hypersensitivity of adult rats with neonatal maternal deprivation (NMD). MethodsVisceral hypersensitivity was quantified by abdominal withdrawal reflex threshold to colorectal distension (CRD). Expression of P2X7Rs was determined by qPCR and Western blot. Synaptic transmission in IC was recorded by patch-clamp recording. ResultsThe expression of P2X7Rs and glutamatergic neurotransmission in IC was significantly increased in NMD rats when compared with age-matched controls. Application of BzATP (P2X7R agonist) enhanced the frequency of spontaneous excitatory postsynaptic currents (sEPSC) and miniature excitatory postsynaptic currents (mEPSC) in IC slices of control rats. Application of BBG (P2X7R antagonist) suppressed the frequencies of sEPSC and mEPSC in IC slices of NMD rats. Microinjection of BzATP into right IC significantly decreased CRD threshold in control rats while microinjection of BBG or A438079 into right IC greatly increased CRD threshold in NMD rats. ConclusionData suggested that the enhanced activities of P2X7Rs in IC, likely through a presynaptic mechanism, contributed to visceral hypersensitivity of adult rats with NMD.
基金：
This work was supported by grants from National Natural Science
Foundation of China (31300909 to YX, 81230024 and 81471137
to GYX) and from the Priority Academic Program Development of
Jiangsu Higher Education Institutions of China.
第一作者机构：[1]Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Laboratory of Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou, China
共同第一作者：
通讯作者：
通讯机构：[*]Laboratory of Translational Pain Medicine, Institute of Neuroscience, Soochow University, Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
Ping-An Zhang ,Qi-Ya Xu,Lu Xue ,et al.Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity[J].CNS NEUROSCIENCE & THERAPEUTICS.2017,23(2):145-154.doi:10.1111/cns.12663.
APA：
Ping-An Zhang,,Qi-Ya Xu,Lu Xue,,Hang Zheng,,Jun Yan,...&Guang-Yin Xu.(2017).Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity.CNS NEUROSCIENCE & THERAPEUTICS,23,(2)
MLA：
Ping-An Zhang,,et al."Neonatal Maternal Deprivation Enhances Presynaptic P2X7 Receptor Transmission in Insular Cortex in an Adult Rat Model of Visceral Hypersensitivity".CNS NEUROSCIENCE & THERAPEUTICS 23..2(2017):145-154
