机构：[a]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, Jiangsu Province, China[b]Department of Neurosurgery, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu Province, China
B23 (also known as Nucleophosmin, NPM, numatrin or NO38) is a ubiquitously expressed phosphoprotein belonging to the nucleoplasmin family of chaperones. In this study we intended to investigate the clinical significance of B23 expression in human glioma and its biological function in glioma cells. Western blot and immunohistochemistry analysis showed that B23 was overexpressed in glioma tissues and glioma cell lines. In addition, the expression level of B23 was positively correlated with glioma pathological grade and Ki-67 expression. Kaplan-Meier analysis revealed that a higher B23 expression in patients with glioma was associated with a poorer prognosis. In vitro, after the release of glioma cell lines from serum starvation, the expression of B23 was upregulated, as well as PCNA (Proliferating Cell Nuclear Antigen) and cyclin A. In addition, knockdown of B23 by small interfering RNA transfection diminished the expression of PCNA, cyclin D1 and arrested cell growth at G1 phase. Taken together, our results implied that B23 could be a candidate prognostic biomarker as well as a potential therapeutical target of glioma. (C) 2015 Elsevier Inc. All rights reserved.
第一作者机构：[a]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, Jiangsu Province, China[b]Department of Neurosurgery, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu Province, China
通讯作者：
通讯机构：[a]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215004, Jiangsu Province, China
推荐引用方式(GB/T 7714)：
Jianguo Chen,Jie Sun,Liu Yang,et al.Upregulation of B23 promotes tumor cell proliferation and predicts poor prognosis in glioma[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2015,466(1):124-30.doi:10.1016/j.bbrc.2015.08.118.
APA：
Jianguo Chen,Jie Sun,Liu Yang,Yaohua Yan,Wei Shi...&Qing Lan.(2015).Upregulation of B23 promotes tumor cell proliferation and predicts poor prognosis in glioma.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,466,(1)
MLA：
Jianguo Chen,et al."Upregulation of B23 promotes tumor cell proliferation and predicts poor prognosis in glioma".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 466..1(2015):124-30
