机构:[1]Capital Med Univ, China Natl Clin Res Ctr Neurol Dis, Beijing Tian Tan Hosp, Dept Neurosurg, 6 Tiantan Xili, Beijing 100050, Peoples R China重点科室诊疗科室国家神经系统疾病临床医学研究中心神经外科神经外科国家神经系统疾病临床医学研究中心首都医科大学附属天坛医院
The human leukocyte antigen f-associated transcript 10 (FAT10) has a similar structure and function with ubiquitin, which efficiently mediate proteasome degradation in an ubiquitin-independent manner. FAT10 expression is upregulated in many tumor tissues and plays a vital role in cell cycle regulation and tumor genesis. However, its role in glioma has not been illuminated. The aim of this study was to evaluate the prognostic value of FAT10 and investigate its functional roles in glioma. The expression of FAT10 in glioma patient samples was examined using quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR), Western blotting and immunohistochemistry methods. Glioma cell lines with either FAT10 overexpression or knockdown were created. The effect of FAT10 on glioma cell migration and invasion was investigated using these cells. In the present study, we had shown that FAT10 was elevated significantly in glioma samples and correlated with tumor pathological grade. FAT10 high-expression glioma is associated with a poor clinical prognosis. Overexpression of FAT10 promoted proliferation, invasion, migration, and sphere formation of glioma cells, whereas downregulation of FAT10 had an opposite effect. Overexpression of FAT10 also promoted the growth of glioma cells in vivo. Moreover, FAT10 enhanced the phosphorylation of Smad2, which contributes to FAT10-induced oncogenic activities in glioma. In conclusion, these findings indicate that FAT10 is a critical regulator potential therapeutic target of glioma.
基金:
National Key Technology Research and Development Program of the Ministry of Science and Technology of ChinaNational Key Technology R&D Program [2013BAI09B03]
第一作者机构:[1]Capital Med Univ, China Natl Clin Res Ctr Neurol Dis, Beijing Tian Tan Hosp, Dept Neurosurg, 6 Tiantan Xili, Beijing 100050, Peoples R China
通讯作者:
通讯机构:[1]Capital Med Univ, China Natl Clin Res Ctr Neurol Dis, Beijing Tian Tan Hosp, Dept Neurosurg, 6 Tiantan Xili, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714):
Dai Bin,Zhang Yisong,Zhang Peng,et al.Upregulation of p-Smad2 contributes to FAT10-induced oncogenic activities in glioma[J].TUMOR BIOLOGY.2016,37(7):8621-8631.doi:10.1007/s13277-015-4739-6.
APA:
Dai, Bin,Zhang, Yisong,Zhang, Peng,Pan, Changcun,Xu, Cheng...&Zhang, Liwei.(2016).Upregulation of p-Smad2 contributes to FAT10-induced oncogenic activities in glioma.TUMOR BIOLOGY,37,(7)
MLA:
Dai, Bin,et al."Upregulation of p-Smad2 contributes to FAT10-induced oncogenic activities in glioma".TUMOR BIOLOGY 37..7(2016):8621-8631