机构：[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China首都医科大学宣武医院[2]Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China[3]Brownell- Talbot School, Omaha, NE, USA[4]Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI, USA[5]Department of Neurosurgery and Brain Repair, University of South Florida, Tampa, FL, USA[6]Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China血管外科首都医科大学宣武医院[7]Department of Neurosurgery Xuanwu Hospital, Capital Medical University, Beijing 100053, China神经外科首都医科大学宣武医院
Platelet-rich plasma (PRP) is a milieu of bioactive factors, including platelet derived growth factor, transforming growth factor beta, among many others. Despite accumulating evidence on PRP's safety and efficacy for treating musculoskeletal injuries, limited studies have been performed using PRP in brain disorders. This study aimed to explore the potential benefits of administration of human PRP lysate after ischemic stroke in rats. An ischemic stroke model was generated by occlusion of the right middle cerebral artery, then 90 min later, stroke rats were randomly assigned to receive local infusion to the ischemic area of human PRP lysate, human albumin solution (HSA), saline or no treatment at all. An additional group of stroke rats received systemic infusion of human PRP lysate to further assess the therapeutic effects of this treatment. Results showed that while local infusion of HSA or saline, and systemic administration of human PRP lysate, compared to no treatment significantly reduced infarct volume (37.4%, 40.1%, and 39.9% vs 49.7%) and neurological deficit score (2.2, 2.6, and 2.8 vs 3.7), the greatest neuroprotection (31.0% infarct volume and 1.6 neurological deficit score) was found in stroke animals that received local intra-arterial infusion of human PRP lysate (p's <0.05). In conclusion, administration of human PRP attenuates brain injury after focal ischemia. Our results suggest PRP should be investigated further as a potential point-of-care biomaterial following stroke. (C) 2014 Elsevier B.V. All rights reserved.
基金：
the National Basic ResearchProgram of China (2011CB707804),
National Science Fundfor Distinguished Young Scholars of China (81325007).
Wethank Jinhuan Gaoand Ning Li (Institute of Hypoxia Medi-cine, Xuanwu Hospital) for technical assistance.
第一作者机构：[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China[2]Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China
通讯作者：
通讯机构：[*1]Capital Medical University,Xuanwu Hospital,No.45,Changchun Street,Xicheng District,Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Ying Zhang ,Guomin Ying,Changhong Ren,et al.Administration of human platelet-rich plasma reduces infarction volume and improves motor function in adult rats with focal ischemic stroke[J].BRAIN RESEARCH.2015,1594:267-273.doi:10.1016/j.brainres.2014.10.035.
APA：
Ying Zhang,,Guomin Ying,Changhong Ren,Yunneng Jizhang,David Brogan...&Xunming Ji,.(2015).Administration of human platelet-rich plasma reduces infarction volume and improves motor function in adult rats with focal ischemic stroke.BRAIN RESEARCH,1594,
MLA：
Ying Zhang,,et al."Administration of human platelet-rich plasma reduces infarction volume and improves motor function in adult rats with focal ischemic stroke".BRAIN RESEARCH 1594.(2015):267-273
