机构：[a]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of China Capital Medical University, and Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China老年医学科神经变性病教育部重点实验室首都医科大学宣武医院[b]Department of Human Anatomy, Sapienza University, Rome 00161, Italy[c]Research Center “Daniel Bovet,” Sapienza University, Rome 00161, Italy .
This study provides the first immunohistochemical evidence of the presence and distribution patterns in the rat spinal cord of alpha-synuclein (alpha-Syn), a soluble acidic protein, widely expressed in the CNS and closely associated to the pathogenesis of neurodegenerative conditions such as Parkinson's and Alzheimer's diseases. We used two novel homemade monoclonal antibodies (2E3 and 3D5) recognizing two different epitopes of Phi-Syn. Both antibodies localized alpha-Syn within the nerve terminals, whereas 3D5 alone also localized it within the neuronal nuclei. alpha-Syn-immunoreactive nervous elements were widely recognized throughout rat spinal cord and in almost all the gray matter laminae. However, they appeared particularly concentrated within laminae 1, 11, VII and X and more scattered in the others. Double immunofluorescent labeling showed that alpha-Syn colocalized with synaptophysin in the presynaptic nerve terminals, with neuropeptide Y (NPY) in lamina I, II, IX and X, and had close relationships with tyrosine hydroxylase (TH) immunoreactive neurons in laminae VII and X. Interestingly, the alpha-Syn-immunoreactive nerve elements, in lamina X, contained little of calbindin-28KD and calretinin-31KD. Our findings could help in understanding the genesis of some early clinical symptoms of Parkinson's disease (PD), such as pain and dysautonomic disorders, and indicate the spinal cord as their probable starting point, according to the ascending theory of PD, proposed by Braak. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of China (30570646 and 30430280), National High-Tech R and D Program of China (2006AA02A408), Natural Science Foundation of Beijing (7022011), Funding Project for Academic Human Resources Development in Institutions of Higher Learning under Jurisdiction of Beijing Municipality PHR (IHLB),
and Italian Ministry for the University and Research (MIUR) and Sapienza University, Rome (T.G.R., Ateneo 2005–2007, Facoltà 2006).
第一作者机构：[a]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of China Capital Medical University, and Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China[b]Department of Human Anatomy, Sapienza University, Rome 00161, Italy[c]Research Center “Daniel Bovet,” Sapienza University, Rome 00161, Italy .
通讯作者：
通讯机构：[a]Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital of China Capital Medical University, and Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China
推荐引用方式(GB/T 7714)：
G. VIVACQUA ,J.-J. YIN ,A. CASINI ,et al.IMMUNOLOCALIZATION OF ALPHA-SYNUCLEIN IN THE RAT SPINAL CORD BY TWO NOVEL MONOCLONAL ANTIBODIES[J].NEUROSCIENCE.2009,158(4):1478-1487.doi:10.1016/j.neuroscience.2008.12.001.
APA：
G. VIVACQUA,,J.-J. YIN,,A. CASINI,,X. LI,,Y.-H. LI,...&S. YU.(2009).IMMUNOLOCALIZATION OF ALPHA-SYNUCLEIN IN THE RAT SPINAL CORD BY TWO NOVEL MONOCLONAL ANTIBODIES.NEUROSCIENCE,158,(4)
MLA：
G. VIVACQUA,,et al."IMMUNOLOCALIZATION OF ALPHA-SYNUCLEIN IN THE RAT SPINAL CORD BY TWO NOVEL MONOCLONAL ANTIBODIES".NEUROSCIENCE 158..4(2009):1478-1487
医学