Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.
基金：
Australian Research Council Centre of Excellence for Integrative Brain Function (ARC Centre)Australian Research Council [CE140100007]; National Health and Medical Research Council of AustraliaNational Health and Medical Research Council of Australia [1027387]
第一作者机构：[1]Univ New South Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW, Australia;
通讯作者：
通讯机构：[1]Univ New South Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW, Australia;[5]Neurosci Res Australia, 139 Barker St, Randwick, NSW 2031, Australia;[6]Univ New South Wales, Sch Med Sci, Kensington, NSW, Australia
推荐引用方式(GB/T 7714)：
Liu Xiaoyun,Xie Beibei,Qi Yanfei,et al.The expression of SEIPIN in the mouse central nervous system[J].BRAIN STRUCTURE & FUNCTION.2016,221(8):4111-4127.doi:10.1007/s00429-015-1151-3.
APA：
Liu, Xiaoyun,Xie, Beibei,Qi, Yanfei,Du, Ximing,Wang, Shaoshi...&Liang, Huazheng.(2016).The expression of SEIPIN in the mouse central nervous system.BRAIN STRUCTURE & FUNCTION,221,(8)
MLA：
Liu, Xiaoyun,et al."The expression of SEIPIN in the mouse central nervous system".BRAIN STRUCTURE & FUNCTION 221..8(2016):4111-4127
