机构：[1]Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China神经变性病教育部重点实验室首都医科大学宣武医院[2]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
(1) Morroniside belongs to an extensive group of natural iridorid glycosides. In the present study, using human neuroblastoma SH-SY5Y cells, we have investigated the protective effects of this compound on modifications in endogenous reduced glutathione (GSH), intracellular oxygen species (ROS) and apoptotic death on H2O2-mediated cytoxicity. (2) Incubation of cells with morroniside led to a significant dose-dependent elevation of cellular GSH accompanied by a marked protection against H2O2-mediated toxicity. Morroniside at 1-100 mu M inhibited the formation of ROS and the activation of caspase-3 and 9, and the upregulation of Bcl-2, whereas no significant change occurred in Bax levels. (3) The results indicated that the anti-oxidative and anti-apoptotic properties render this natural compound potentially protective against H2O2-induced cytotoxicity. (4) This study suggested that intracellular GSH appeared to be an important factor in morroniside-mediated cytoprotection against H2O2-toxicity in SH-SY5Y cells.
基金：
Acknowledgement This research was supported by the grants from the Program Foundation: Chinese ‘‘973’’ program foundation (2003CB517104); Beijing Natural Science Foundation, China (No: 7062031, 7050001, 7032013); Beijing Science and Technology Committee Program Foundation, China (D0204003000031, D0206001043191); Beijing Superiority Talented Man Foundation, China (No: 2004100501816); Base-Clinical Foundation of Capital Medical University, China (No: 2004JL02, 2006JL09); Key Laboratory for Neurodegenerative Disease Ministry of Education Foundation, China (No:2007).
第一作者机构：[1]Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
通讯作者：
通讯机构：[1]Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Wen Wang,Wenting Huang,Lin Li,et al.Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells[J].CELLULAR AND MOLECULAR NEUROBIOLOGY.2008,28(2):293-305.doi:10.1007/s10571-007-9168-7.
APA：
Wen Wang,Wenting Huang,Lin Li,Houxi Ai,Fangling Sun...&Yi An.(2008).Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells.CELLULAR AND MOLECULAR NEUROBIOLOGY,28,(2)
MLA：
Wen Wang,et al."Morroniside prevents peroxide-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells".CELLULAR AND MOLECULAR NEUROBIOLOGY 28..2(2008):293-305
