机构：[1]Department of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen, China,[2]Department of Microbial Research, WeHealthGene Institute, Shenzhen, China,[3]Department of Computer Science, City University of Hong Kong, Hong Kong, China,[4]Department of Respiratory Diseases, Beijing Children’s Hospital, Beijing, China,临床科室呼吸科首都医科大学附属北京儿童医院[5]Institute of Statistics, NanKai University, Tianjin, China,[6]Department of Pediatrics, Shenzhen Dapeng District Maternity and Child Healthcare Hospital, Shenzhen, China
Community-acquired pneumonia (CAP) is a worldwide infectious disease caused by bacteria, viruses, or a combination of these infectious agents. Mycoplasma pneumoniae is an atypical pneumonia pathogen that causes high morbidity and mortality in children, and adenovirus can lead to severe pneumonia. However, the etiology of different types of pneumonia is still unclear. In this study, we selected a total of 52 inpatients with M. pneumoniae pneumonia (MPP) (n = 21), adenovirus pneumonia (AVP) (n = 16), or tracheomalacia (n = 15) to serve as a disease control. Bronchoalveolar lavage fluid (BALF) samples that had been obtained for clinical use were analyzed. We compared the differences in microbiota and the expression of 10 inflammatory cytokines in samples between MPP, AVP, and tracheomalacia. We found that the bacterial diversity in MPP was lower than that in AVP and tracheomalacia. Mycoplasma, Streptococcus, and Pseudomonas were predominant in samples of MPP, AVP, and tracheomalacia, respectively. The expression levels of IL-6, IL-8, and IL-10 were significantly higher in inpatients with AVP compared to children hospitalized with tracheomalacia or MPP. The lung microbiota in MPP was remarkably correlated with IL-2, IL-4, IL-5, IL-6, TNF-alpha, and IL-1 alpha expressions, while this was not found in tracheomalacia and AVP. Microbiota analysis identified a high load of multi-drug resistant Acinetobacter baumannii in the lung microbiota of several inpatients, which might be associated with the long hospitalization length and intra-group differences at the individual level. This study will help to understand the microbial etiology of tracheomalacia, AVP, and MPP and to identify effective therapies for these diseases.
基金：
Key Medical Disciplines Building Project of Shenzhen [SZXJ2017005]; Sanming Project of Medicine in Shenzhen [SZSM201512030]; Shenzhen public service platform for clinical drug trials [20151964]; Shenzhen Science and Technology Project [JCYJ20170303155012371]
第一作者机构：[1]Department of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Respiratory Diseases, Shenzhen Children’s Hospital, Shenzhen, China,[2]Department of Microbial Research, WeHealthGene Institute, Shenzhen, China,[5]Institute of Statistics, NanKai University, Tianjin, China,
推荐引用方式(GB/T 7714)：
Wang Heping,Zhou Qian,Dai Wenkui,et al.Lung Microbiota and Pulmonary Inflammatory Cytokines Expression Vary in Children With Tracheomalacia and Adenoviral or Mycoplasma pneumoniae Pneumonia[J].FRONTIERS IN PEDIATRICS.2019,7(JUN):-.doi:10.3389/fped.2019.00265.
APA：
Wang, Heping,Zhou, Qian,Dai, Wenkui,Feng, Xin,Lu, Zhiwei...&Zheng, Yuejie.(2019).Lung Microbiota and Pulmonary Inflammatory Cytokines Expression Vary in Children With Tracheomalacia and Adenoviral or Mycoplasma pneumoniae Pneumonia.FRONTIERS IN PEDIATRICS,7,(JUN)
MLA：
Wang, Heping,et al."Lung Microbiota and Pulmonary Inflammatory Cytokines Expression Vary in Children With Tracheomalacia and Adenoviral or Mycoplasma pneumoniae Pneumonia".FRONTIERS IN PEDIATRICS 7..JUN(2019):-
