Previously research shows the scar tissue formed in the injured area after spinal cord injury blocks nerve regeneration and functional recovery. However, the researchers tried to prevent the formation of scar after spinal cord injury to promote nerve regeneration, but it ran counter to their desire, indicating the formation of scar might play roles on functional recovery after spinal cord injury.To investigate roles of scar formation on functional repair after spinal cord injury, we selected several different key time points to resect the scar tissue formed after spinal cord injury based on the rat models of the T8-T9 transection injury of spinal cord. Firstly, the recovery of motor function was evaluated by BBB score and electrophysiological examination; secondly, the pathological features of functional recovery were analyzed mainly by immunofluorescence βⅢ-tubulin staining; finally, the genes related to the recovery of motor function were predicted by high-throughput sequencing analysis.Immunofluorescence results showed that the resection of scar tissue promoted significantly the recovery of motor function and the expression of βⅢ-tubulin in the injured area in the second week after spinal cord injury. Furthermore, RNA-seq studies showed that Tubb3, Tubb6 genes expression and other neural regeneration pathways were significantly different in the tissue before and after early resection.Excision of scar tissue in the second week promoted nerve regeneration after spinal cord injury. Tubb3 and Tubb6 genes might be the potential targets for spinal cord injury therapy in our study.Copyright © 2022 Elsevier Inc. All rights reserved.