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An efficient protocol for studying human pluripotent stem cell-derived myotube senescence

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资源类型：
Pubmed体系：

收录情况： ◇ CSCD-C

作者：
Zhao Qian[1,2] * ; Jing Ying[1,2] * ; Ma Shuai[4,6,8] ; Zhang Weiqi[5,7,8,9,10] ; Qu Jing[3,5,6,8] ; Wang Si[1,2] ; Liu Guang-Hui[1,2,4,5,6,8] ;
机构： [1]Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China [2]Aging Translational Medicine Center, International Center for Aging and Cancer, Beijing Municipal Geriatric Medical Research Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China [3]State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [4]State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [5]University of Chinese Academy of Sciences, Beijing 100049, China [6]Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China [7]CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics and China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China [8]Institute for Stem Cell and Regeneration, CAS, Beijing 100101, China [9]Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, China [10]Sino-Danish Center for Education and Research, Beijing 101408, China
出处：
DOI： 10.52601/bpr.2023.230013
ISSN：

关键词： Human pluripotent stem cell Myotube Senescence Differentiation In vitro

摘要：
Sarcopenia, an age-related skeletal muscle condition characterized by a progressive decline in muscle mass and function, is linked to increased vulnerability, a higher likelihood of falls, and higher mortality rates in older individuals. A comprehensive understanding of the intricate mechanisms driving skeletal muscle aging is of great significance in both scientific and clinical fields. Consequently, myotube models that facilitate studying regulatory mechanisms underlying skeletal muscle aging are important tools required to advance intervention strategies against skeletal muscle aging and associated disorders. Here, we provide a detailed protocol to generate human pluripotent stem cells-derived myotubes and describe their applications in aging studies, as well as a troubleshooting for potential problems. Overall, this protocol serves as a valuable methodological reference for exploring the role and mechanism of genes involved in skeletal muscle aging.© The Author(s) 2023.

基金：
We greatly thank Xiuping Li, Jing Lu, Ying Yang, and Lei Bai for their administrative assistance. This work was supported by the National Natural Science Foundation of China (82071588, 82122024), the National Key Research and Development Program of China (2022YFA1103700,2020YFA0804000, 2020YFA0112200, 2021YFF1201000, the STI2030-Major Projects-2021ZD0202400), the National Natural Science Foundation of China (82001477, 81921006, 82125011, 92149301, 92168201, 91949209, 92049304, 92049116, 32121001, 82192863, 82122024, 32000500, 82271600), CAS Project for Young Scientists in Basic Research (YSBR-076, YSBR- 012), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16000000), the Program of the Beijing Natural Science Foundation (Z190019), the Informatization Plan of Chinese Academy of Sciences (CASWX2021SF- 0301, CAS-WX2022SDC-XK14, CAS-WX2021SF- 0101), New Cornerstone Science Foundation through the XPLORER PRIZE (2021-1045), Youth Innovation Promotion Association of CAS (E1CAZW0401, 2022083), Excellent Young Talents Program of Capital Medical University (12300927), The Project for Technology Development of Beijing-affiliated Medical Research Institutes (11000023T000002036310), Excellent Young Talents Training Program for the Construction of Beijing Municipal University Teacher Team (BPHR202203105), Young Elite Scientists Sponsorship Program by CAST (YESS20210002), and Beijing Hospitals Authority Youth Program (QML20230806).
语种：
外文
PubmedID：
第一作者：
第一作者机构： [1]Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China [2]Aging Translational Medicine Center, International Center for Aging and Cancer, Beijing Municipal Geriatric Medical Research Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
共同第一作者：
通讯作者：
通讯机构： [1]Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China [2]Aging Translational Medicine Center, International Center for Aging and Cancer, Beijing Municipal Geriatric Medical Research Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China [3]State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [4]State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [5]University of Chinese Academy of Sciences, Beijing 100049, China [6]Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China [8]Institute for Stem Cell and Regeneration, CAS, Beijing 100101, China
推荐引用方式(GB/T 7714)：
Zhao Qian,Jing Ying,Ma Shuai,et al.An efficient protocol for studying human pluripotent stem cell-derived myotube senescence[J].Biophysics Reports.2023,9(5):232-240.doi:10.52601/bpr.2023.230013.
APA：
Zhao Qian,Jing Ying,Ma Shuai,Zhang Weiqi,Qu Jing...&Liu Guang-Hui.(2023).An efficient protocol for studying human pluripotent stem cell-derived myotube senescence.Biophysics Reports,9,(5)
MLA：
Zhao Qian,et al."An efficient protocol for studying human pluripotent stem cell-derived myotube senescence".Biophysics Reports 9..5(2023):232-240

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