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The phosphorylation of CHK1 at Ser345 regulates the phenotypic switching of vascular smooth muscle cells both in vitro and in vivo

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机构： [a]General Department of Xuan Wu Hospital Capital Medical University, Beijing, 100053, China [b]Vascular Surgery Department of Xuan Wu Hospital Capital Medical University, Institute of Vascular Sutgery, Capital Medical University, Beijing, 100053, China [c]Beijing Institute of Brain Disorders, Capital Medical University, Beijing, 100069, China

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关键词： Artery stenosis CHK1 DNA damage Intimal hyperplasia Phenotype

摘要：

Background and aims: DNA damage and repair have been shown to be associated with carotid artery restenosis and atherosclerosis. The proliferation and migration of vascular smooth muscle cells (VSMCs) is the main cause of artery stenosis. This study aims to define the relationship between DNA damage and VSMCs proliferation. Methods: A rat carotid artery injury model was established, and human and rat VSMCs cultured in vitro. H2O2 was used to induce DNA damage in vitro. The selected CHK1 inhibitor, LY2603618, was used to inhibit CHK1 phosphorylation both in vivo and in vitro. γH2AX, αSMA and phosphorylated CHK1 were detected both in rat carotid artery and cultured VSMCs from different groups. Hyperplasia ratio of rat carotid artery intimal was measured. Results: DNA double-strand breaks occur in the rat carotid artery after injury. DNA damage induces CHK1 phosphorylation and down-regulates αSMA expression in VSMCs both in vitro and in vivo. The inhibition of CHK1 phosphorylation rescues αSMA expression in VSMCs both in vitro and in vivo, and rat carotid intimal hyperplasia after injury was suppressed. Conclusions: Our data demonstrated that phosphorylation of CHK1 under DNA damage stress modulates VSMCs phenotypic switching. CHK1 inhibition may be a potential therapeutic strategy for intima hyperplasia treatment. © 2020 Elsevier B.V.

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出版当年[2019]版：

大类 | 2 区医学

小类 | 2 区外周血管病 3 区心脏和心血管系统

最新[2023]版：

大类 | 2 区医学

小类 | 2 区外周血管病 3 区心脏和心血管系统

JCR分区：

出版当年[2018]版：

Q1 PERIPHERAL VASCULAR DISEASE Q2 CARDIAC & CARDIOVASCULAR SYSTEMS

最新[2023]版：

Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Q1 PERIPHERAL VASCULAR DISEASE

影响因子： 4.9 最新[2023版] 5.3 最新五年平均 4.255 出版当年[2018版] 4.239 出版当年五年平均 4.467 出版前一年[2017版] 3.919 出版后一年[2019版]

第一作者：

第一作者机构： [a]General Department of Xuan Wu Hospital Capital Medical University, Beijing, 100053, China [b]Vascular Surgery Department of Xuan Wu Hospital Capital Medical University, Institute of Vascular Sutgery, Capital Medical University, Beijing, 100053, China

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